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LysoBrite™ Red DND-99

Chemical structure for LysoBrite™ Red DND-99.
Chemical structure for LysoBrite™ Red DND-99.
Ordering information
Price ()
Catalog Number22647
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight399.25
SolventDMSO
Spectral properties
Excitation (nm)573
Emission (nm)592
Storage, safety and handling
Certificate of OriginDownload PDF
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
399.25
Excitation (nm)
573
Emission (nm)
592
LysoBrite Red DND-99 is chemically same to the LysoTracker® Red DND-99 used for labeling and tracking acidic organelles in live cells (LysoTracker® is the trademark of ThermoFisher). It has good selectivity for acidic organelles, and reasonably retaining its staining pattern after aldehyde fixation. The LysoBrite™ probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH, allowing them to freely permeate cell membranes to label live cells.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of LysoBrite™ Red DND-99 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM250.47 µL1.252 mL2.505 mL12.523 mL25.047 mL
5 mM50.094 µL250.47 µL500.939 µL2.505 mL5.009 mL
10 mM25.047 µL125.235 µL250.47 µL1.252 mL2.505 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)573
Emission (nm)592

Citations


View all 61 citations: Citation Explorer
Quantitation of Lysosomal Trapping of Basic Lipophilic Compounds Using In Vitro Assays and In Silico Predictions Based on the Determination of the Full pH Profile of the Endo-/Lysosomal System in Rat Hepatocytes
Authors: Schmitt, M. V., Lienau, P., Fricker, G., Reichel, A.
Journal: Drug Metab Dispos (2019): 49-57
Detection and discrimination of Shigella sonnei and Shigella flexneri based on vacuolar responses in Saccharomyces cerevisiae
Authors: Nguyen, N. T., Park, R. M., Kim, Y. H., Min, J.
Journal: J Biotechnol (2018): 1-7
Transport and release of colloidal 3-mercaptopropionic acid-coated CdSe-CdS/ZnS core-multishell quantum dots in human umbilical vein endothelial cells
Authors: Fontana, J. M., Yin, H., Chen, Y., Florez, R., Brismar, H., Fu, Y.
Journal: Int J Nanomedicine (2017): 8615-8629
An autophagic process is activated in HepG2 cells to mediate BDE-100-induced toxicity
Authors: Pereira, L. C., Duarte, F. V., Varela, A. T., Rolo, A. P., Palmeira, C. M., Dorta, D. J.
Journal: Toxicology (2017): 59-65
Aggregatibacter actinomycetemcomitans leukotoxin induces cytosol acidification in LFA-1 expressing immune cells
Authors: Balashova, N., Dhingra, A., Boesze-Battaglia, K., Lally, E. T.
Journal: Mol Oral Microbiol (2016): 106-14
Bafilomycin A1 Attenuates Osteoclast Acidification and Formation, Accompanied by Increased Levels of SQSTM1/p62 Protein
Authors: Zhu, S., Rea, S. L., Cheng, T., Feng, H. T., Walsh, J. P., Ratajczak, T., Tickner, J., Pavlos, N., Xu, H. Z., Xu, J.
Journal: J Cell Biochem (2016): 1464-70
Rab11 and Lysotracker Markers Reveal Correlation between Endosomal Pathways and Transfection Efficiency of Surface-Functionalized Cationic Liposome-DNA Nanoparticles
Authors: Majzoub, R. N., Wonder, E., Ewert, K. K., Kotamraju, V. R., Teesalu, T., Safinya, C. R.
Journal: J Phys Chem B (2016): 6439-53
Presence of an isoform of H+-pyrophosphatase located in the alveolar sacs of a scuticociliate parasite of turbot: physiological consequences
Authors: Mallo, N., Lamas, J., Defelipe, A. P., Decastro, M. E., Sueiro, R. A., Leiro, J. M.
Journal: Parasitology (2016): 576-87
Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities
Authors: Harlan, F. K., Lusk, J. S., Mohr, B. M., Guzikowski, A. P., Batchelor, R. H., Jiang, Y., Naleway, J. J.
Journal: PLoS One (2016): e0156312
Intravital Imaging Reveals Angiotensin II-Induced Transcytosis of Albumin by Podocytes
Authors: Schiessl, I. M., Hammer, A., Kattler, V., Gess, B., Theilig, F., Witzgall, R., Castrop, H.
Journal: J Am Soc Nephrol (2016): 731-44