Name: Mag-520™ AM
Brand: AAT Bioquest
SKU: 20406
Price: 348 USD
Availability: InStock

Mag-520™ AM

Intracellular magnesium is important for mediating enzymatic reactions, DNA synthesis, hormone secretion, and muscle contraction. A vast majority of the existing magnesium ion indicators are based on tricarboxylate APTRA chelator derived from the popular tetracarboxylate BAPTA calcium ion chelator. They include mag-fura-2, mag-indo-1, mag-fluo-4 and mag-rhod-3. However, all of them have higher affinity for calcium than magnesium although they were designed for detecting magnesium ion. Mag-520™ is the first commercial magnesium indicator that has higher affinity for magnesium than calcium. Its significantly improved selectivity can be used for magnesium signaling applications with fluorescence microscopy or flow cytometry. Mag-520™ AM is cell-permeable with almost identical spectra to those of FITC, making it a convenient fluorescent probe used with FITC filter set that is ubiquitously equipped with almost all fluorescence instruments.

In vitro detection of Mg<sup>+2</sup>. HL-60 cells were stained with AM form of Mag-520™ (5 uM) and mag-fluo-4 (5 uM) for 30 minutes and stimulated with FCCP (20 uM) for 30 minutes and response was recorded for end point analysis. Response was recorded using NovoCyte™ 3000 Flow Cytometer with 530/30 nm filter and 488 nm laser.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20406	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Mag-520™ AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	172.574 µL	862.872 µL	1.726 mL	8.629 mL	17.257 mL
5 mM	34.515 µL	172.574 µL	345.149 µL	1.726 mL	3.451 mL
10 mM	17.257 µL	86.287 µL	172.574 µL	862.872 µL	1.726 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	579.46 g/mol	=	mL	x	mM	=	mmol

Citations

View all 15 citations: Citation Explorer

A highly selective fluorescent probe for the intracellular measurement of magnesium ion

Authors:

Patel, Deven and Peng, Ruogu and Guo, Haitao and Lin, Raechel and Zhao, Qin and Liao, Jinfang and Diwu, Zhenjun

Journal:

Analytical Biochemistry (2020): 113910

A Highly Selective Green Fluorescent Magnesium Indicator for Intracellular Magnesium Ion Analysis

Authors:

Patel, Deven and Zhao, Qin and Guo, Haitao and Peng, Ruogu and Liu, Jixiang and Liao, Jinfang and Diwu, Zhenjun

Journal:

Biophysical Journal (2020): 273a

Fluorescence lifetime imaging of intracellular magnesium content in live cells

Authors:

Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.

Journal:

Analyst (2019): 1876-1880

Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium

Authors:

Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.

Journal:

Nat Protoc (2017): 461-471

A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples

Authors:

Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.

Journal:

Analyst (2014): 1201-7

Physical properties

Molecular weight	579.46
Solvent	DMSO

Spectral properties

Excitation (nm)	506
Emission (nm)	525

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on September 23, 2025