Mag-520™ potassium salt

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	511.51
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	507
Emission (nm)	531

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-520™ potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	195.5 µL	977.498 µL	1.955 mL	9.775 mL	19.55 mL
5 mM	39.1 µL	195.5 µL	390.999 µL	1.955 mL	3.91 mL
10 mM	19.55 µL	97.75 µL	195.5 µL	977.498 µL	1.955 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	507
Emission (nm)	531

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Cal-520®, potassium salt	492	515	0.75¹
Calbryte™ 520, potassium salt	493	515	0.75¹

Images

Figure 1. Fluorescence intensity at 525 nm (arbitrary units, excitation at 490 nm) of Mag-520 in the presence of Mg2+(red) or Ca2+ (blue) ranging from 0.05 to 300 mM. All intensity were measured in 50 mM HEPES buffer (pH=7.2)

Citations

View all 13 citations: Citation Explorer

Fluorescence lifetime imaging of intracellular magnesium content in live cells
Authors: Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880

Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471

A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7

Intracellular magnesium detection by fluorescent indicators
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44

Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7

Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kudo, S., Kitamura, Y., Suzuki, K., Oka, K.
Journal: Biochim Biophys Acta (2005): 19-28

Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kitamura, Y., Suzuki, K., Oka, K.
Journal: J Am Coll Nutr (2004): 742S-4S

Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging
Authors: Komatsu, H., Iwasawa, N., Citterio, D., Suzuki, Y., Kubota, T., Tokuno, K., Kitamura, Y., Oka, K., Suzuki, K.
Journal: J Am Chem Soc (2004): 16353-60

Fluorescent monitoring of Jurkatt cell intracellular magnesium during metabolic poisoning
Authors: Carroll, R. G., Thomas, J. M.
Journal: Shock (1994): 213-6

Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1
Authors: Morelle, B., Salmon, J. M., Vigo, J., Viallet, P.
Journal: Anal Biochem (1994): 170-6