Mag-520™ potassium salt

A vast majority of the existing magnesium ion indicators are based on tricarboxylate APTRA chelator derived from the popular tetracarboxylate BAPTA calcium ion chelator. They include mag-fura-2, mag-indo-1, mag-fluo-4 and mag-rhod-3. However, all of them have higher affinity for calcium than magnesium although they were designed for detecting magnesium ion. Mag-520™ is the first commercial magnesium indicator that has higher affinity for magnesium than calcium. Its significantly improved selectivity can be used for measuring magnesium ion with minimal interference from calcium ion compared to other commercial magnesium ion indicators such as the popular mag-fluo-4.

Fluorescence intensity at 525 nm (arbitrary units, excitation at 490 nm) of Mag-520 in the presence of Mg2+(red) or Ca2+ (blue) ranging from 0.05 to 300 mM. All intensity were measured in 50 mM HEPES buffer (pH=7.2)

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20404	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-520™ potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	195.5 µL	977.498 µL	1.955 mL	9.775 mL	19.55 mL
5 mM	39.1 µL	195.5 µL	390.999 µL	1.955 mL	3.91 mL
10 mM	19.55 µL	97.75 µL	195.5 µL	977.498 µL	1.955 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	511.51 g/mol	=	mL	x	mM	=	mmol

Citations

View all 13 citations: Citation Explorer

Fluorescence lifetime imaging of intracellular magnesium content in live cells

Authors:

Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.

Journal:

Analyst (2019): 1876-1880

Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium

Authors:

Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.

Journal:

Nat Protoc (2017): 461-471

A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples

Authors:

Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.

Journal:

Analyst (2014): 1201-7

Intracellular magnesium detection by fluorescent indicators

Authors:

Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.

Journal:

Methods Enzymol (2012): 421-44

Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism

Authors:

Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.

Journal:

Tanpakushitsu Kakusan Koso (2007): 1552-7

Physical properties

Molecular weight	511.51
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	507
Emission (nm)	531

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 22, 2025