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Mag-520™ potassium salt

A vast majority of the existing magnesium ion indicators are based on tricarboxylate APTRA chelator derived from the popular tetracarboxylate BAPTA calcium ion chelator. They include mag-fura-2, mag-indo-1, mag-fluo-4 and mag-rhod-3. However, all of them have higher affinity for calcium than magnesium although they were designed for detecting magnesium ion. Mag-520™ is the first commercial magnesium indicator that has higher affinity for magnesium than calcium. Its significantly improved selectivity can be used for measuring magnesium ion with minimal interference from calcium ion compared to other commercial magnesium ion indicators such as the popular mag-fluo-4.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-520™ potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM195.5 µL977.498 µL1.955 mL9.775 mL19.55 mL
5 mM39.1 µL195.5 µL390.999 µL1.955 mL3.91 mL
10 mM19.55 µL97.75 µL195.5 µL977.498 µL1.955 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Quantum yield
Cal-520®, potassium salt4925150.751
Calbryte™ 520, potassium salt4935150.751

Citations

View all 13 citations: Citation Explorer
Fluorescence lifetime imaging of intracellular magnesium content in live cells
Authors: Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880
Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471
A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7
Intracellular magnesium detection by fluorescent indicators
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44
Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7
Page updated on November 1, 2024

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Catalog Number20404
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Physical properties

Molecular weight

511.51

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

507

Emission (nm)

531

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure
Fluorescence intensity at 525 nm (arbitrary units, excitation at 490 nm) of Mag-520 in the presence of Mg2+(red) or Ca2+ (blue) ranging from 0.05 to 300 mM.  All intensity were measured in 50 mM HEPES buffer (pH=7.2)
Fluorescence intensity at 525 nm (arbitrary units, excitation at 490 nm) of Mag-520 in the presence of Mg2+(red) or Ca2+ (blue) ranging from 0.05 to 300 mM.  All intensity were measured in 50 mM HEPES buffer (pH=7.2)
Fluorescence intensity at 525 nm (arbitrary units, excitation at 490 nm) of Mag-520 in the presence of Mg2+(red) or Ca2+ (blue) ranging from 0.05 to 300 mM.  All intensity were measured in 50 mM HEPES buffer (pH=7.2)