Mag-Fluo-4 potassium salt

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Dissociation constant (K_d, nM)	22000
Molecular weight	681.77
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	495
Emission (nm)	528
Quantum yield	0.16¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

681.77

Dissociation constant (K_d, nM)

22000

Extinction coefficient (cm ^-1 M ^-1)

82000

Excitation (nm)

495

Emission (nm)

528

Quantum yield

0.16¹

The cell-impermeant Mag-Fluo-4 is an analog of Fluo-4 with a Kd for Mg ion of 4.7 mM and a Kd for Ca ion of 22 µM, making it useful as an intracellular Mg ion indicator as well as a low-affinity Ca ion indicator.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-Fluo-4 potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	146.677 µL	733.385 µL	1.467 mL	7.334 mL	14.668 mL
5 mM	29.335 µL	146.677 µL	293.354 µL	1.467 mL	2.934 mL
10 mM	14.668 µL	73.339 µL	146.677 µL	733.385 µL	1.467 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	495
Emission (nm)	528
Quantum yield	0.16¹

Images

Figure 1. Chemical structure for Mag-Fluo-4 potassium salt

References

View all 25 references: Citation Explorer

Real-time intra-store confocal Ca2+ imaging in isolated mouse cardiomyocytes
Authors: Fern, undefined and ez-Tenorio M, Niggli E.
Journal: Cell Calcium. (2016)

Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead
Authors: Wang H, Wang ZK, Jiao P, Zhou XP, Yang DB, Wang ZY, Wang L.
Journal: Toxicology (2015): 137

Role of Mitofusin-2 in mitochondrial localization and calcium uptake in skeletal muscle
Authors: Ainbinder A, Boncompagni S, Protasi F, Dirksen RT.
Journal: Cell Calcium (2015): 14

TRPC1 is involved in Ca(2)(+) influx and cytotoxicity following Pb(2)(+) exposure in human embryonic kidney cells
Authors: Zhang H, Li W, Xue Y, Zou F.
Journal: Toxicol Lett (2014): 52

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach
Authors: Calderon JC, Bolanos P, Caputo C.
Journal: J Muscle Res Cell Motil (2014): 279

EGF stimulates Mg(2+) influx in mammary epithelial cells
Authors: Trapani V, Arduini D, Luongo F, Wolf FI.
Journal: Biochem Biophys Res Commun (2014): 572

High-throughput functional assays of IP3-evoked Ca2+ release
Authors: Tovey SC, Taylor CW.
Journal: Cold Spring Harb Protoc (2013): 930

Presence of store-operated Ca2+ entry in C57BL/6J mouse ventricular myocytes and its suppression by sevoflurane
Authors: Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
Journal: Br J Anaesth (2012): 352

Measurement and simulation of myoplasmic calcium transients in mouse slow-twitch muscle fibres
Authors: Hollingworth S, Kim MM, Baylor SM.
Journal: J Physiol (2012): 575

TRPC1, STIM1, and ORAI influence signal-regulated intracellular and endoplasmic reticulum calcium dynamics in human myometrial cells
Authors: Murtazina DA, Chung D, Ulloa A, Bryan E, Galan HL, Sanborn BM.
Journal: Biol Reprod (2011): 315