Mag-Fura-2, AM *Cell-permeant*

Mag-Fura-2, AM

Cell-permeant

Mag-Fura-2, AM is an intracellular magnesium indicator that is ratiometric and UV light-excitable. It has the spectral properties that closely match Fura-2. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading. It is also used for measuring high level of calcium ion in live cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20383	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Mag-Fura-2, AM *Cell-permeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	138.397 µL	691.984 µL	1.384 mL	6.92 mL	13.84 mL
5 mM	27.679 µL	138.397 µL	276.794 µL	1.384 mL	2.768 mL
10 mM	13.84 µL	69.198 µL	138.397 µL	691.984 µL	1.384 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	722.56 g/mol	=	mL	x	mM	=	mmol

References

View all 15 references: Citation Explorer

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues

Authors:

Zhang G, Jacquemin D, Buccella D.

Journal:

J Phys Chem B. (2017)

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica

Authors:

Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.

Journal:

FEMS Microbiol Lett (2004): 49

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC

Authors:

Hyrc KL, Bownik JM, Goldberg MP.

Journal:

Cell Calcium (2000): 75

Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+

Authors:

Martinez-Zaguilan R, Parnami J, Martinez GM.

Journal:

Cell Physiol Biochem (1998): 158

Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes

Authors:

Kennedy HJ., undefined

Journal:

Exp Physiol (1998): 449

Physical properties

Molecular weight	722.56
Solvent	DMSO

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	130100-20-8

Instrument settings

Fluorescence microscope
Excitation	Fura 2 filter set
Emission	Fura 2 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	340, 380
Emission	510
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on September 29, 2025