Mag-Fura-2 tetrapotassium salt Cell impermeant

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Physical properties

Molecular weight	586.68
Solvent	Water

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

132319-57-4

Molecular weight

586.68

Excitation (nm)

336

Emission (nm)

505

Mag-Fura-2, tetrapotassium salt is an intracellular magnesium indicator that is ratiometric and UV light-excitable. This water-soluble salt form is useful for intracellular loading by microinjection or infusion from patch pipette. This particular magnesium indicator is also used for high concentration calcium detection.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Mag-Fura-2 tetrapotassium salt *Cell impermeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	170.451 µL	852.253 µL	1.705 mL	8.523 mL	17.045 mL
5 mM	34.09 µL	170.451 µL	340.901 µL	1.705 mL	3.409 mL
10 mM	17.045 µL	85.225 µL	170.451 µL	852.253 µL	1.705 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Excitation (nm)	336
Emission (nm)	505

Images

Figure 1. Chemical structure for Mag-Fura-2 tetrapotassium salt *Cell impermeant*

References

View all 15 references: Citation Explorer

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75

Characterization of Mg2+ transport in brush border membrane vesicles of rabbit ileum studied with mag-fura-2
Authors: Juttner R, Ebel H.
Journal: Biochim Biophys Acta (1998): 51

Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ., undefined
Journal: Exp Physiol (1998): 449

Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158

Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189

Intrasynaptosomal free Mg2+ concentration measured with the fluorescent indicator mag-fura-2: modulation by Na+ gradient and by extrasynaptosomal ATP
Authors: Garcia-Martin E, Martin-Romero FJ, Gutierrez-Merino C.
Journal: J Neurochem (1995): 2757

Effects of monovalent cations, pH and temperature on the dissociation constant (KD) for the fluorescent indicator mag-fura-2 at different excitation wavelengths
Authors: Howarth FC, Singh J, Waring JJ, Hustler BI, Bailey M.
Journal: Magnes Res (1995): 299

Interactions of polyamines in the measurement of free magnesium concentration by mag-fura-2 and 31P-NMR
Authors: Gunther T, Vormann J, Konstanczak P, Schafer A.
Journal: Biochim Biophys Acta (1994): 281