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MagaDye™ 588-ddTTP

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Physical properties
Molecular weight~1900
Spectral properties
Absorbance (nm)498
Excitation (nm)498
Emission (nm)588
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Excitation (nm)
Emission (nm)
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. The four distinct fluorescent ddNTPs (labeled with BigDye®, BigDye® is the trademark of ThermoFisher) are the critical components for performing Sanger sequencing. MagaDye™ 588-ddTTP is equivalent to BigDye dTMR color with almost identical spectra.


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Spectral properties

Absorbance (nm)498
Excitation (nm)498
Emission (nm)588



View all 50 references: Citation Explorer
Update on molecular companion diagnostics - a future in personalized medicine beyond Sanger sequencing.
Authors: Campbell, Michelle Renee
Journal: Expert review of molecular diagnostics (2020)
Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.
Authors: Cermak, Nathan and Datta, Manoshi Sen and Conwill, Arolyn
Journal: iScience (2020): 100915
Guidelines for Sanger sequencing and molecular assay monitoring.
Authors: Crossley, Beate M and Bai, Jianfa and Glaser, Amy and Maes, Roger and Porter, Elizabeth and Killian, Mary Lea and Clement, Travis and Toohey-Kurth, Kathy
Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagn (2020): 1040638720905833
A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next‑generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523
Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)
Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
Authors: Chen, Nan-Yu and Kao, Shu-Wei and Liu, Zhuo-Hao and Wu, Ting-Shu and Tsai, Chia-Lung and Lin, Hsi-Hsun and Wong, Wing-Wai and Chang, Yea-Yuan and Chen, Shu-Sheng and Ku, Stephane Wen-Wei
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2020): 182-191
Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)
Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing.
Authors: Fu, Ya and Zeng, Yongbin and Chen, Tianbin and Chen, Huijuan and Lin, Ni and Lin, Jinpiao and Liu, Xiaofeng and Huang, Er and Wu, Songhang and Wu, Shu and Xu, Siyi and Wang, Long and Ou, Qishui
Journal: Journal of clinical microbiology (2019)
The Prevalence, Anatomic Distribution and Significance of HPV Genotypes in Head and Neck Squamous Papillomas as Detected by Real-Time PCR and Sanger Sequencing.
Authors: Trzcinska, A and Zhang, W and Gitman, M and Westra, W H
Journal: Head and neck pathology (2019)
Full mtDNA genome sequencing of Brazilian admixed populations: A forensic-focused evaluation of a MPS application as an alternative to Sanger sequencing methods.
Authors: Avila, E and Graebin, P and Chemale, G and Freitas, J and Kahmann, A and Alho, C S
Journal: Forensic science international. Genetics (2019): 154-164