Maleimide Activated mKLH *Optimized for Maximum Immunization Response*

Maleimide Activated mKLH

Optimized for Maximum Immunization Response

Maleimide Activated mKLH is engineered to facilitate the conjugation of sulfhydryl-containing peptide haptens to the highly immunogenic keyhole limpet hemocyanin (KLH) carrier protein, optimizing it for immunization and antibody production. This high-purity, mariculture-derived KLH is chemically modified with a Sulfo-SMCC crosslinker, rendering it reactive to sulfhydryl groups (-SH) on cysteine residues of peptides and other thiol-containing haptens. This modification enables the conjugation of multiple antigen molecules to each KLH molecule, significantly enhancing immunogenicity and increasing the likelihood of eliciting high-titer antisera in immunized subjects. The conjugation reaction with cysteine-containing peptides is performed at pH 6.6-7.0, and the extent of conjugation can be determined by a colorimetric assay using Ellman’s reagent to detect unreacted thiol groups. Maleimide-activated mKLH is supplied in a lyophilized, ready-to-use form, eliminating the need for time-consuming activation and purification steps.

General packaging for Cat. No. 5612. Your actual packaging may differ.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
5612	5 x 1 mg	Price

References

View all 20 references: Citation Explorer

Preparation of isodesmosine-KLH conjugate for ELISA system.

Authors:

Baut, Daria A and Tanaka, Nao and Yokoo, Reiko and Usuki, Toyonobu

Journal:

Chirality (2020): 431-436

Development of Functional Antibodies Directed to Human Dialyzable Leukocyte Extract (Transferon®).

Authors:

Mellado-Sánchez, Gabriela and Lázaro-Rodríguez, Juan José and Avila, Sandra and Vallejo-Castillo, Luis and Vázquez-Leyva, Said and Carballo-Uicab, Gregorio and Velasco-Velázquez, Marco and Medina-Rivero, Emilio and Pavón, Lenin and Chacón-Salinas, Rommel and Pérez-Tapia, Sonia Mayra

Journal:

Journal of immunology research (2019): 2754920

Making Weak Antigens Strong: Cross-Linking Peptides to KLH with Maleimide.

Authors:

Greenfield, Edward A and DeCaprio, James and Brahmandam, Mohan

Journal:

Cold Spring Harbor protocols (2018)

Preclinical Efficacy and Characterization of Candidate Vaccines for Treatment of Opioid Use Disorders Using Clinically Viable Carrier Proteins.

Authors:

Baruffaldi, Federico and Kelcher, April Huseby and Laudenbach, Megan and Gradinati, Valeria and Limkar, Ajinkya and Roslawski, Michaela and Birnbaum, Angela and Lees, Andrew and Hassler, Carla and Runyon, Scott and Pravetoni, Marco

Journal:

Molecular pharmaceutics (2018): 4947-4962

Synthesis of an S-Linked α(2→8) GD3 Antigen and Evaluation of the Immunogenicity of Its Glycoconjugate.

Authors:

Kuan, Ting-Chun and Wu, Hsin-Ru and Adak, Avijit K and Li, Ben-Yuan and Liang, Chien-Fu and Hung, Jung-Tung and Chiou, Shih-Pin and Yu, Alice L and Hwu, Jih Ru and Lin, Chun-Cheng

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2017): 6876-6887

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12171501

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Page updated on April 2, 2026