mBBr [Monobromobimane] *CAS 71418-44-5*

mBBr

Monobromobimane; CAS 71418-44-5

mBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. mBBr is used for the determination of the redox status of low molecular weight and protein thiols in biological systems. MBBr-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and bromobimane accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy following protein precipitation.

Catalog	Size	Price	Quantity
633	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mBBr [Monobromobimane] *CAS 71418-44-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	368.854 µL	1.844 mL	3.689 mL	18.443 mL	36.885 mL
5 mM	73.771 µL	368.854 µL	737.708 µL	3.689 mL	7.377 mL
10 mM	36.885 µL	184.427 µL	368.854 µL	1.844 mL	3.689 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	271.11 g/mol	=	mL	x	mM	=	mmol

References

View all 25 references: Citation Explorer

Reactivity of zinc finger cysteines: chemical modifications within labile zinc fingers in estrogen receptor

Authors:

Atsriku C, Scott GK, Benz CC, Baldwin MA.

Journal:

J Am Soc Mass Spectrom (2005): 2017

Interaction of S100A8/S100A9-arachidonic acid complexes with the scavenger receptor CD36 may facilitate fatty acid uptake by endothelial cells

Authors:

Kerkhoff C, Sorg C, T and on NN, Nacken W.

Journal:

Biochemistry (2001): 241

Determination of plasma homocysteine by high performance liquid chromatography with fluorescence detection

Authors:

Liao Y, Liang YQ, Zhi XM, Liao ZH.

Journal:

Se Pu (2000): 49

Plasma cysteine deficiency and decreased reduction of nitrososulfamethoxazole with HIV infection

Authors:

Naisbitt DJ, Vilar FJ, Stalford AC, Wilkins EG, Pirmohamed M, Park BK.

Journal:

AIDS Res Hum Retroviruses (2000): 1929

Fatty acid translocase/CD36 mediates the uptake of palmitate by type II pneumocytes

Authors:

Guthmann F, Haupt R, Looman AC, Spener F, Rustow B.

Journal:

Am J Physiol (1999): L191

Physical properties

Molecular weight	271.11
Solvent	DMSO

Spectral properties

Excitation (nm)	395
Emission (nm)	490

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	71418-44-5

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Page updated on October 13, 2025