Name: mBCl [Monochlorobimane] *CAS 76421-73-3*
Brand: AAT Bioquest
SKU: 635
Price: 115 USD
Availability: InStock

mBCl

Monochlorobimane; CAS 76421-73-3

Monochloromobimane (mBCl) is essentially nonfluorescent until reacted with a thiol group. It readily reacts with several low molecular weight thiols, including cysteine, glutathione, N-acetylcysteine, mercaptopurine, peptides and plasma thiols. The glutathione conjugate of mBCl has absorption/emission maxima ~394/490 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
635	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mBCl [Monochlorobimane] *CAS 76421-73-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	441.189 µL	2.206 mL	4.412 mL	22.059 mL	44.119 mL
5 mM	88.238 µL	441.189 µL	882.379 µL	4.412 mL	8.824 mL
10 mM	44.119 µL	220.595 µL	441.189 µL	2.206 mL	4.412 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	226.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Morphological profiling reveals neuroprotection via mitochondrial uncoupling in human dopaminergic neurons

Authors:

Gorgogietas, Vyron and Weiss, Am{\'e}lie and Cousin, Lo{\"\i}c and Hoffmann, David and Schmitt, Karen and Ogier, Arnaud and Barbuti, Peter A and Santos, Bruno FR and Boussaad, Ibrahim and Wittich, Annika and others,

Journal:

Scientific Reports (2025): 29507

Combined structural and functional imaging of the kidney reveals major axial differences in proximal tubule endocytosis

Authors:

Schuh, Claus D and Polesel, Marcello and Platonova, Evgenia and Haenni, Dominik and Gassama, Alkaly and Tokonami, Natsuko and Ghazi, Susan and Bugarski, Milica and Devuyst, Olivier and Ziegler, Urs and others,

Journal:

Journal of the American Society of Nephrology (2018): 2696--2712

References

View all 19 references: Citation Explorer

Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes

Authors:

Webb C, Bedwell C, Guth A, Avery P, Dow S.

Journal:

Vet Immunol Immunopathol (2006): 129

Formation and rapid export of the monochlorobimane-glutathione conjugate in cultured rat astrocytes

Authors:

Waak J, Dringen R.

Journal:

Neurochem Res (2006): 1409

Effects of monochlorobimane on cerebral ischemia-induced damage to mitochondria

Authors:

Oshikawa S, Miyake-Takagi K, Takagi N, Abe T, Kuruhara Y, Takeo S.

Journal:

Biol Pharm Bull (2004): 166

Measurement of the intracellular distribution of reduced glutathione in cultured rat hepatocytes using monochlorobimane and confocal laser scanning microscopy

Authors:

Stevenson D, Wokosin D, Girkin J, Grant MH.

Journal:

Toxicol In Vitro (2002): 609

Fluorometric microplate assay to measure glutathione S-transferase activity in insects and mites using monochlorobimane

Authors:

Nauen R, Stumpf N.

Journal:

Anal Biochem (2002): 194

Physical properties

Molecular weight	226.66
Solvent	DMSO

Spectral properties

Excitation (nm)	389
Emission (nm)	483

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	76421-73-3

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Page updated on October 12, 2025