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mBCl [Monochlorobimane] *CAS 76421-73-3*

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Physical properties
Molecular weight226.66
SolventDMSO
Spectral properties
Excitation (nm)389
Emission (nm)483
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

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CAS
76421-73-3
Molecular weight
226.66
Excitation (nm)
389
Emission (nm)
483
Monochloromobimane (mBCl) is essentially nonfluorescent until reacted with a thiol group. It readily reacts with several low molecular weight thiols, including cysteine, glutathione, N-acetylcysteine, mercaptopurine, peptides and plasma thiols. The glutathione conjugate of mBCl has absorption/emission maxima ~394/490 nm.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mBCl [Monochlorobimane] *CAS 76421-73-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM441.189 µL2.206 mL4.412 mL22.059 mL44.119 mL
5 mM88.238 µL441.189 µL882.379 µL4.412 mL8.824 mL
10 mM44.119 µL220.595 µL441.189 µL2.206 mL4.412 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)389
Emission (nm)483

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Citations


View all 1 citations: Citation Explorer
Combined structural and functional imaging of the kidney reveals major axial differences in proximal tubule endocytosis
Authors: Schuh, Claus D and Polesel, Marcello and Platonova, Evgenia and Haenni, Dominik and Gassama, Alkaly and Tokonami, Natsuko and Ghazi, Susan and Bugarski, Milica and Devuyst, Olivier and Ziegler, Urs and others,
Journal: Journal of the American Society of Nephrology (2018): 2696--2712

References


View all 19 references: Citation Explorer
Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes
Authors: Webb C, Bedwell C, Guth A, Avery P, Dow S.
Journal: Vet Immunol Immunopathol (2006): 129
Formation and rapid export of the monochlorobimane-glutathione conjugate in cultured rat astrocytes
Authors: Waak J, Dringen R.
Journal: Neurochem Res (2006): 1409
Effects of monochlorobimane on cerebral ischemia-induced damage to mitochondria
Authors: Oshikawa S, Miyake-Takagi K, Takagi N, Abe T, Kuruhara Y, Takeo S.
Journal: Biol Pharm Bull (2004): 166
Measurement of the intracellular distribution of reduced glutathione in cultured rat hepatocytes using monochlorobimane and confocal laser scanning microscopy
Authors: Stevenson D, Wokosin D, Girkin J, Grant MH.
Journal: Toxicol In Vitro (2002): 609
Fluorometric microplate assay to measure glutathione S-transferase activity in insects and mites using monochlorobimane
Authors: Nauen R, Stumpf N.
Journal: Anal Biochem (2002): 194
Screening for recombinant glutathione transferases active with monochlorobimane
Authors: Eklund BI, Edalat M, Stenberg G, Mannervik B.
Journal: Anal Biochem (2002): 102
Efflux of glutathione conjugate of monochlorobimane from striatal and cortical neurons
Authors: DeCory HH, Piech-Dumas KM, Sheu SS, Federoff HJ, Anders MW.
Journal: Drug Metab Dispos (2001): 1256
Quantitative imaging of glutathione in hippocampal neurons and glia in culture using monochlorobimane
Authors: Keelan J, Allen NJ, Antcliffe D, Pal S, Duchen MR.
Journal: J Neurosci Res (2001): 873
Monochlorobimane fluorometric method to measure tissue glutathione
Authors: Kamencic H, Lyon A, Paterson PG, Juurlink BH.
Journal: Anal Biochem (2000): 35
Glutathione levels in primary glial cultures: monochlorobimane provides evidence of cell type-specific distribution
Authors: Chatterjee S, Noack H, Possel H, Keilhoff G, Wolf G.
Journal: Glia (1999): 152