MCA [7-Methoxycoumarin-4-acetic acid] CAS 62935-72-2

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Physical properties

Molecular weight	234.2
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.3
Excitation (nm)	322
Emission (nm)	381

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

62935-72-2

Molecular weight

234.2

Correction Factor (280 nm)

0.3

Excitation (nm)

322

Emission (nm)

381

7-Methoxycoumarin-4-acetic acid (MCA) is widely used for developing FRET peptide substrates for analyzing protease activities. It is commonly paired with dinitrophenyl (DNP) group. It is also used for HPLC derivatization by fluorescence detection.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCA [7-Methoxycoumarin-4-acetic acid] *CAS 62935-72-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	426.985 µL	2.135 mL	4.27 mL	21.349 mL	42.699 mL
5 mM	85.397 µL	426.985 µL	853.971 µL	4.27 mL	8.54 mL
10 mM	42.699 µL	213.493 µL	426.985 µL	2.135 mL	4.27 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.3
Excitation (nm)	322
Emission (nm)	381

Images

Figure 1. Chemical structure for MCA [7-Methoxycoumarin-4-acetic acid] *CAS 62935-72-2*

References

View all 6 references: Citation Explorer

Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306

Fluorescent labeling of peptides on solid phase
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582

Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470

Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131

Analysis of 5-fluorouracil in plasma by precolumn derivatization with 4-bromomethyl-7-methoxycoumarin, followed by multi-dimensional high-performance liquid chromatography
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431