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MCA [7-Methoxycoumarin-4-acetic acid] *CAS 62935-72-2*

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Physical properties
Molecular weight234.2
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Coumarins
CAS
62935-72-2
Molecular weight
234.2
Correction Factor (280 nm)
0.3
Excitation (nm)
322
Emission (nm)
381
7-Methoxycoumarin-4-acetic acid (MCA) is widely used for developing FRET peptide substrates for analyzing protease activities. It is commonly paired with dinitrophenyl (DNP) group. It is also used for HPLC derivatization by fluorescence detection.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCA [7-Methoxycoumarin-4-acetic acid] *CAS 62935-72-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM426.985 µL2.135 mL4.27 mL21.349 mL42.699 mL
5 mM85.397 µL426.985 µL853.971 µL4.27 mL8.54 mL
10 mM42.699 µL213.493 µL426.985 µL2.135 mL4.27 mL

Molarity calculator

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Spectrum


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Spectral properties

Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381

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References


View all 6 references: Citation Explorer
Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods
Authors: Lesner A, Wysocka M, Guzow K, Wiczk W, Legowska A, Rolka K.
Journal: Anal Biochem (2008): 306
Fluorescent labeling of peptides on solid phase
Authors: Katritzky AR, Yoshioka M, Narindoshvili T, Chung A, Johnson JV.
Journal: Org Biomol Chem (2008): 4582
Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin
Authors: Ciencialova A, Zakova L, Jiracek J, Barthova J, Barth T.
Journal: J Pept Sci (2004): 470
Substrate binding of gelatinase B induces its enzymatic activity in the presence of intact propeptide
Authors: Bannikov GA, Karelina TV, Collier IE, Marmer BL, Goldberg GI.
Journal: J Biol Chem (2002): 16022
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Analysis of 5-fluorouracil in plasma by precolumn derivatization with 4-bromomethyl-7-methoxycoumarin, followed by multi-dimensional high-performance liquid chromatography
Authors: Kindberg CG, Riley CM, Stobaugh JF, Slavik M.
Journal: J Chromatogr (1989): 431