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Mca-PLAQAV-Dpa-RSSSR-NH2
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Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorogenic peptide substrate for detecting tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. Its N-terminal contains a highly fluorescent 7-methoxycoumarin (MCA) fluorophore that is efficiently quenched by non-fluorescent 2,4-dinitrophenyl group on Dpa via fluorescence resonance energy transfer (dark FRET). It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, resulting an increase in fluorescence. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between A (Ala) and V (Val) residues.
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References
View all 13 references: Citation Explorer
Increased plasma TACE activity in subjects with mild cognitive impairment and patients with Alzheimer's disease.
Authors: Sun, Qiying and Hampel, Harald and Blennow, Kaj and Lista, Simone and Levey, Allan and Tang, Beisha and Li, Rena and Shen, Yong
Journal: Journal of Alzheimer's disease : JAD (2014): 877-86
Authors: Sun, Qiying and Hampel, Harald and Blennow, Kaj and Lista, Simone and Levey, Allan and Tang, Beisha and Li, Rena and Shen, Yong
Journal: Journal of Alzheimer's disease : JAD (2014): 877-86
Primary structure and characterization of a non hemorrhagic metalloproteinase with fibrinolytic activity, from the snake venom of Protobothrops tokarensis (Tokara-habu).
Authors: Oyama, Etsuko and Kitagawa, Yasuyuki and Takahashi, Hidenobu
Journal: Toxicon : official journal of the International Society on Toxinology (2013): 153-61
Authors: Oyama, Etsuko and Kitagawa, Yasuyuki and Takahashi, Hidenobu
Journal: Toxicon : official journal of the International Society on Toxinology (2013): 153-61
Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren's syndrome salivary glands.
Authors: Sisto, Margherita and Lisi, Sabrina and Lofrumento, Dario Domenico and Ingravallo, Giuseppe and Mitolo, Vincenzo and D'Amore, Massimo
Journal: Histochemistry and cell biology (2010): 345-53
Authors: Sisto, Margherita and Lisi, Sabrina and Lofrumento, Dario Domenico and Ingravallo, Giuseppe and Mitolo, Vincenzo and D'Amore, Massimo
Journal: Histochemistry and cell biology (2010): 345-53
ADAM-17: the enzyme that does it all.
Authors: Gooz, Monika
Journal: Critical reviews in biochemistry and molecular biology (2010): 146-69
Authors: Gooz, Monika
Journal: Critical reviews in biochemistry and molecular biology (2010): 146-69
Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
Authors: Huang, Yi-Wei and Su, Paul and Liu, Guang Ying and Crow, Min Rui and Chaukos, Deanna and Yan, Harry and Robinson, Lisa A
Journal: The Journal of biological chemistry (2009): 29644-53
Authors: Huang, Yi-Wei and Su, Paul and Liu, Guang Ying and Crow, Min Rui and Chaukos, Deanna and Yan, Harry and Robinson, Lisa A
Journal: The Journal of biological chemistry (2009): 29644-53