AAT Bioquest


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Physical properties
Molecular weight1638.96
Spectral properties
Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorogenic peptide substrate for detecting tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. Its N-terminal contains a highly fluorescent 7-methoxycoumarin (MCA) fluorophore that is efficiently quenched by non-fluorescent 2,4-dinitrophenyl group on Dpa via fluorescence resonance energy transfer (dark FRET). It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, resulting an increase in fluorescence. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between A (Ala) and V (Val) residues.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Mca-PLAQAV-Dpa-RSSSR-NH2 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM61.014 µL305.072 µL610.143 µL3.051 mL6.101 mL
5 mM12.203 µL61.014 µL122.029 µL610.143 µL1.22 mL
10 mM6.101 µL30.507 µL61.014 µL305.072 µL610.143 µL

Molarity calculator

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Spectral properties

Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381



View all 13 references: Citation Explorer
Increased plasma TACE activity in subjects with mild cognitive impairment and patients with Alzheimer's disease.
Authors: Sun, Qiying and Hampel, Harald and Blennow, Kaj and Lista, Simone and Levey, Allan and Tang, Beisha and Li, Rena and Shen, Yong
Journal: Journal of Alzheimer's disease : JAD (2014): 877-86
Primary structure and characterization of a non hemorrhagic metalloproteinase with fibrinolytic activity, from the snake venom of Protobothrops tokarensis (Tokara-habu).
Authors: Oyama, Etsuko and Kitagawa, Yasuyuki and Takahashi, Hidenobu
Journal: Toxicon : official journal of the International Society on Toxinology (2013): 153-61
ADAM-17: the enzyme that does it all.
Authors: Gooz, Monika
Journal: Critical reviews in biochemistry and molecular biology (2010): 146-69
Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren's syndrome salivary glands.
Authors: Sisto, Margherita and Lisi, Sabrina and Lofrumento, Dario Domenico and Ingravallo, Giuseppe and Mitolo, Vincenzo and D'Amore, Massimo
Journal: Histochemistry and cell biology (2010): 345-53
Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases.
Authors: Huang, Yi-Wei and Su, Paul and Liu, Guang Ying and Crow, Min Rui and Chaukos, Deanna and Yan, Harry and Robinson, Lisa A
Journal: The Journal of biological chemistry (2009): 29644-53
Drug insight: tumor necrosis factor-converting enzyme as a pharmaceutical target for rheumatoid arthritis.
Authors: Moss, Marcia L and Sklair-Tavron, Liora and Nudelman, Raphael
Journal: Nature clinical practice. Rheumatology (2008): 300-9
Relationships between early inflammatory response to bleomycin and sensitivity to lung fibrosis: a role for dipeptidyl-peptidase I and tissue inhibitor of metalloproteinase-3?
Authors: Pottier, Nicolas and Chupin, Cécile and Defamie, Virginie and Cardinaud, Bruno and Sutherland, Rachel and Rios, Géraldine and Gauthier, Francis and Wolters, Paul J and Berthiaume, Yves and Barbry, Pascal and Mari, Bernard
Journal: American journal of respiratory and critical care medicine (2007): 1098-107
Photic injury promotes cleavage of p75NTR by TACE and nuclear trafficking of the p75 intracellular domain.
Authors: Srinivasan, Bhooma and Wang, Zhaohui and Brun-Zinkernagel, Anne M and Collier, Robert J and Black, Roy A and Frank, Stuart J and Barker, Philip A and Roque, Rouel S
Journal: Molecular and cellular neurosciences (2007): 449-61
Expressions of tumor necrosis factor-converting enzyme and ErbB3 in rats with chronic obstructive pulmonary disease.
Authors: Ju, Chun-rong and Xia, Xi-zheng and Chen, Rong-chang
Journal: Chinese medical journal (2007): 1505-10
Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme.
Authors: Neumann, Ulf and Kubota, Hisashi and Frei, Karl and Ganu, Vishwas and Leppert, David
Journal: Analytical biochemistry (2004): 166-73