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Mca-YVADAPK(Dnp)-OH

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Physical properties
Molecular weight1145.15
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Coumarins
Molecular weight
1145.15
Correction Factor (280 nm)
0.3
Excitation (nm)
322
Emission (nm)
381
Mca-YVADAPK(Dnp)-OH is a fluorogenic peptide substrate for detecting caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2). Its N-terminal contains a highly fluorescent 7-methoxycoumarin (MCA) fluorophore that is efficiently quenched by non-fluorescent 2,4-dinitrophenyl group on lysine residue via fluorescence resonance energy transfer (dark FRET). It can be used to measure the activities of caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2) that are capable of cleaving an amide bond between the fluorescent group (MCA) and the quencher group (DNP), resulting an increase in fluorescence.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Mca-YVADAPK(Dnp)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.325 µL436.624 µL873.248 µL4.366 mL8.732 mL
5 mM17.465 µL87.325 µL174.65 µL873.248 µL1.746 mL
10 mM8.732 µL43.662 µL87.325 µL436.624 µL873.248 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.3
Excitation (nm)322
Emission (nm)381

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References


View all 50 references: Citation Explorer
Marked Up-Regulation of ACE2 in Hearts of Patients With Obstructive Hypertrophic Cardiomyopathy: Implications for SARS-CoV-2-Mediated COVID-19.
Authors: Bos, J Martijn and Hebl, Virginia B and Oberg, Ann L and Sun, Zhifu and Herman, Daniel S and Teekakirikul, Polakit and Seidman, Jonathan G and Seidman, Christine E and Dos Remedios, Cristobal G and Maleszewski, Joseph J and Schaff, Hartzell V and Dearani, Joseph A and Noseworthy, Peter A and Friedman, Paul A and Ommen, Steve R and Brozovich, Frank V and Ackerman, Michael J
Journal: Mayo Clinic proceedings (2020): 1354-1368
ACE2 expression in adipose tissue is associated with COVID-19 cardio-metabolic risk factors and cell type composition.
Authors: El-Sayed Moustafa, Julia Sarah and Jackson, Anne U and Brotman, Sarah M and Guan, Li and Villicaňa, Sergio and Roberts, Amy L and Zito, Antonino and Bonnycastle, Lori and Erdos, Michael R and Narisu, Narisu and Stringham, Heather M and Welch, Ryan and Yan, Tingfen and Lakka, Timo and Parker, Stephen and Tuomilehto, Jaakko and Collins, Francis S and Pajukanta, Päivi and Boehnke, Michael and Koistinen, Heikki A and Laakso, Markku and Falchi, Mario and Bell, Jordana T and Scott, Laura J and Mohlke, Karen L and Small, Kerrin S
Journal: medRxiv : the preprint server for health sciences (2020)
ACE2 receptor polymorphism: Susceptibility to SARS-CoV-2, hypertension, multi-organ failure, and COVID-19 disease outcome.
Authors: Devaux, Christian A and Rolain, Jean-Marc and Raoult, Didier
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2020): 425-435
The protein expression profile of ACE2 in human tissues.
Authors: Hikmet, Feria and Méar, Loren and Edvinsson, Åsa and Micke, Patrick and Uhlén, Mathias and Lindskog, Cecilia
Journal: Molecular systems biology (2020): e9610
Expression of SARS-CoV-2 Entry Molecules ACE2 and TMPRSS2 in the Gut of Patients With IBD.
Authors: Burgueño, Juan F and Reich, Adrian and Hazime, Hajar and Quintero, Maria A and Fernandez, Irina and Fritsch, Julia and Santander, Ana M and Brito, Nivis and Damas, Oriana M and Deshpande, Amar and Kerman, David H and Zhang, Lanyu and Gao, Zhen and Ban, Yuguang and Wang, Lily and Pignac-Kobinger, Judith and Abreu, Maria T
Journal: Inflammatory bowel diseases (2020): 797-808
Elevated FiO2 increases SARS-CoV-2 co-receptor expression in respiratory tract epithelium.
Authors: Myti, Despoina and Gunjak, Miša and Casado, Francisco and Khaghani Raziabad, Solmaz and Nardiello, Claudio and Vadász, István and Herold, Susanne and Pryhuber, Gloria and Seeger, Werner and Morty, Rory E
Journal: American journal of physiology. Lung cellular and molecular physiology (2020): L670-L674
Broad host range of SARS-CoV-2 predicted by comparative and structural analysis of ACE2 in vertebrates.
Authors: Damas, Joana and Hughes, Graham M and Keough, Kathleen C and Painter, Corrie A and Persky, Nicole S and Corbo, Marco and Hiller, Michael and Koepfli, Klaus-Peter and Pfenning, Andreas R and Zhao, Huabin and Genereux, Diane P and Swofford, Ross and Pollard, Katherine S and Ryder, Oliver A and Nweeia, Martin T and Lindblad-Toh, Kerstin and Teeling, Emma C and Karlsson, Elinor K and Lewin, Harris A
Journal: Proceedings of the National Academy of Sciences of the United States of America (2020): 22311-22322
Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses.
Authors: Qi, Furong and Qian, Shen and Zhang, Shuye and Zhang, Zheng
Journal: Biochemical and biophysical research communications (2020): 135-140
Autophagy as an emerging target for COVID-19: lessons from an old friend, chloroquine.
Authors: Bonam, Srinivasa Reddy and Muller, Sylviane and Bayry, Jagadeesh and Klionsky, Daniel J
Journal: Autophagy (2020): 1-7
SARS-CoV-2 infection of human ACE2-transgenic mice causes severe lung inflammation and impaired function.
Authors: Winkler, Emma S and Bailey, Adam L and Kafai, Natasha M and Nair, Sharmila and McCune, Broc T and Yu, Jinsheng and Fox, Julie M and Chen, Rita E and Earnest, James T and Keeler, Shamus P and Ritter, Jon H and Kang, Liang-I and Dort, Sarah and Robichaud, Annette and Head, Richard and Holtzman, Michael J and Diamond, Michael S
Journal: Nature immunology (2020)