MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*

MCLA

2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride; CAS 128322-44-1

MCLA has been used to assess superoxide formation in a variety of conditions, including neutrophil and macrophage activation, cultured cells and vascular tissue. By using MCLA chemiluminescence, in vivo superoxide production has been reported from the surface of liver, intestine, heart and lung. MCLA-amplified chemiluminescence is also used to determine the extracellular superoxide production from spermatozoa.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15250	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	342.783 µL	1.714 mL	3.428 mL	17.139 mL	34.278 mL
5 mM	68.557 µL	342.783 µL	685.565 µL	3.428 mL	6.856 mL
10 mM	34.278 µL	171.391 µL	342.783 µL	1.714 mL	3.428 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	291.73 g/mol	=	mL	x	mM	=	mmol

References

View all 44 references: Citation Explorer

Development of imidazopyrazinone red-chemiluminescent probes for detecting superoxide anions via a chemiluminescence resonance energy transfer method

Authors:

Teranishi K., undefined

Journal:

Luminescence. (2006)

Chemiluminescent visualization of superoxide generated by Candida albicans

Authors:

Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.

Journal:

Med Mycol (2004): 427

Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase

Authors:

Okai Y, Sato EF, Higashi-Okai K, Inoue M.

Journal:

Free Radic Biol Med (2004): 1412

Immunoglobulin G induces microglial superoxide production

Authors:

Yoshida T, Tanaka M, Okamoto K.

Journal:

Neurol Res (2002): 361

Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method

Authors:

Sakurai T, Terakawa S.

Journal:

Methods Mol Biol (2002): 203

Physical properties

Molecular weight	291.73
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	128322-44-1

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Page updated on October 8, 2025