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MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*

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Physical properties
Molecular weight291.73
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
MCLA has been used to assess superoxide formation in a variety of conditions, including neutrophil and macrophage activation, cultured cells and vascular tissue. By using MCLA chemiluminescence, in vivo superoxide production has been reported from the surface of liver, intestine, heart and lung. MCLA-amplified chemiluminescence is also used to determine the extracellular superoxide production from spermatozoa.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM342.783 µL1.714 mL3.428 mL17.139 mL34.278 mL
5 mM68.557 µL342.783 µL685.565 µL3.428 mL6.856 mL
10 mM34.278 µL171.391 µL342.783 µL1.714 mL3.428 mL

Molarity calculator

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View all 44 references: Citation Explorer
Development of imidazopyrazinone red-chemiluminescent probes for detecting superoxide anions via a chemiluminescence resonance energy transfer method
Authors: Teranishi K., undefined
Journal: Luminescence. (2006)
Chemiluminescent visualization of superoxide generated by Candida albicans
Authors: Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.
Journal: Med Mycol (2004): 427
Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase
Authors: Okai Y, Sato EF, Higashi-Okai K, Inoue M.
Journal: Free Radic Biol Med (2004): 1412
Immunoglobulin G induces microglial superoxide production
Authors: Yoshida T, Tanaka M, Okamoto K.
Journal: Neurol Res (2002): 361
Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method
Authors: Sakurai T, Terakawa S.
Journal: Methods Mol Biol (2002): 203
Chemiluminescence of superoxide generated by Candida albicans: differential effects of the superoxide generator paraquat on a wild-type strain and a respiratory mutant
Authors: Aoki S, Ito-Kuwa S, Nakamura K, Nakamura Y, Vidotto V, Takeo K.
Journal: Med Mycol (2002): 13
Mechanism of superoxide anion production by hepatic sinusoidal endothelial cells and Kupffer cells during short-term ethanol perfusion in the rat
Authors: Hasegawa T, Kikuyama M, Sakurai K, Kambayashi Y, Adachi M, Saniabadi AR, Kuwano H, Nakano M.
Journal: Liver (2002): 321
In vivo measurement of superoxide in the cerebral cortex during anoxia-reoxygenation and ischemia-reperfusion
Authors: Yamaguchi K, Uematsu D, Itoh Y, Watanabe S, Fukuuchi Y.
Journal: Keio J Med (2002): 201
Continuous observation of superoxide generation in an in-situ ischemia-reperfusion rat lung model
Authors: Midorikawa J, Maehara K, Yaoita H, Watanabe T, Ohtani H, Ushiroda S, Maruyama Y.
Journal: Jpn Circ J (2001): 207
Detection and cytotoxicity of cisplatin-induced superoxide anion in monolayer cultures of a human ovarian cancer cell line
Authors: Masuda H, Tanaka T, Tateishi M, Naito M, Tamai H.
Journal: Cancer Chemother Pharmacol (2001): 155