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MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*
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Application notes
MCLA has been used to assess superoxide formation in a variety of conditions, including neutrophil and macrophage activation, cultured cells and vascular tissue. By using MCLA chemiluminescence, in vivo superoxide production has been reported from the surface of liver, intestine, heart and lung. MCLA-amplified chemiluminescence is also used to determine the extracellular superoxide production from spermatozoa.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 342.783 µL | 1.714 mL | 3.428 mL | 17.139 mL | 34.278 mL |
| 5 mM | 68.557 µL | 342.783 µL | 685.565 µL | 3.428 mL | 6.856 mL |
| 10 mM | 34.278 µL | 171.391 µL | 342.783 µL | 1.714 mL | 3.428 mL |
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References
View all 44 references: Citation Explorer
Development of imidazopyrazinone red-chemiluminescent probes for detecting superoxide anions via a chemiluminescence resonance energy transfer method
Authors: Teranishi K., undefined
Journal: Luminescence. (2006)
Authors: Teranishi K., undefined
Journal: Luminescence. (2006)
Chemiluminescent visualization of superoxide generated by Candida albicans
Authors: Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.
Journal: Med Mycol (2004): 427
Authors: Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.
Journal: Med Mycol (2004): 427
Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase
Authors: Okai Y, Sato EF, Higashi-Okai K, Inoue M.
Journal: Free Radic Biol Med (2004): 1412
Authors: Okai Y, Sato EF, Higashi-Okai K, Inoue M.
Journal: Free Radic Biol Med (2004): 1412
Immunoglobulin G induces microglial superoxide production
Authors: Yoshida T, Tanaka M, Okamoto K.
Journal: Neurol Res (2002): 361
Authors: Yoshida T, Tanaka M, Okamoto K.
Journal: Neurol Res (2002): 361
Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method
Authors: Sakurai T, Terakawa S.
Journal: Methods Mol Biol (2002): 203
Authors: Sakurai T, Terakawa S.
Journal: Methods Mol Biol (2002): 203