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MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*

MCLA has been used to assess superoxide formation in a variety of conditions, including neutrophil and macrophage activation, cultured cells and vascular tissue. By using MCLA chemiluminescence, in vivo superoxide production has been reported from the surface of liver, intestine, heart and lung. MCLA-amplified chemiluminescence is also used to determine the extracellular superoxide production from spermatozoa.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM342.783 µL1.714 mL3.428 mL17.139 mL34.278 mL
5 mM68.557 µL342.783 µL685.565 µL3.428 mL6.856 mL
10 mM34.278 µL171.391 µL342.783 µL1.714 mL3.428 mL

Molarity calculator

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References

View all 44 references: Citation Explorer
Development of imidazopyrazinone red-chemiluminescent probes for detecting superoxide anions via a chemiluminescence resonance energy transfer method
Authors: Teranishi K., undefined
Journal: Luminescence. (2006)
Chemiluminescent visualization of superoxide generated by Candida albicans
Authors: Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.
Journal: Med Mycol (2004): 427
Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase
Authors: Okai Y, Sato EF, Higashi-Okai K, Inoue M.
Journal: Free Radic Biol Med (2004): 1412
Immunoglobulin G induces microglial superoxide production
Authors: Yoshida T, Tanaka M, Okamoto K.
Journal: Neurol Res (2002): 361
Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method
Authors: Sakurai T, Terakawa S.
Journal: Methods Mol Biol (2002): 203
Page updated on November 11, 2024

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Physical properties

Molecular weight

291.73

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

128322-44-1
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