MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*

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Chemical structure for MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1*
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1 mg 15250 $95

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Ex/Em (nm)430/546
CAS #128322-44-1
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Category Enzyme Detection
Redox Enzymes
Related Cell Signaling Molecules
Reactive Oxygen Species
Secondary Reagents
MCLA has been used to assess superoxide formation in a variety of conditions, including neutrophil and macrophage activation, cultured cells and vascular tissue. By using MCLA chemiluminescence, in vivo superoxide production has been reported from the surface of liver, intestine, heart and lung. MCLA-amplified chemiluminescence is also used to determine the extracellular superoxide production from spermatozoa.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MCLA [2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, hydrochloride] *CAS 128322-44-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

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Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
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References & Citations

Development of imidazopyrazinone red-chemiluminescent probes for detecting superoxide anions via a chemiluminescence resonance energy transfer method
Authors: Teranishi K.
Journal: Luminescence. (2006)

Chemiluminescent visualization of superoxide generated by Candida albicans
Authors: Masui S, Majima T, Nakamura K, Ito-Kuwa S, Takeo K, Aoki S.
Journal: Med Mycol (2004): 427

Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase
Authors: Okai Y, Sato EF, Higashi-Okai K, Inoue M.
Journal: Free Radic Biol Med (2004): 1412

Chemiluminescence of superoxide generated by Candida albicans: differential effects of the superoxide generator paraquat on a wild-type strain and a respiratory mutant
Authors: Aoki S, Ito-Kuwa S, Nakamura K, Nakamura Y, Vidotto V, Takeo K.
Journal: Med Mycol (2002): 13

Immunoglobulin G induces microglial superoxide production
Authors: Yoshida T, Tanaka M, Okamoto K.
Journal: Neurol Res (2002): 361

In vivo measurement of superoxide in the cerebral cortex during anoxia-reoxygenation and ischemia-reperfusion
Authors: Yamaguchi K, Uematsu D, Itoh Y, Watanabe S, Fukuuchi Y.
Journal: Keio J Med (2002): 201

Mechanism of superoxide anion production by hepatic sinusoidal endothelial cells and Kupffer cells during short-term ethanol perfusion in the rat
Authors: Hasegawa T, Kikuyama M, Sakurai K, Kambayashi Y, Adachi M, Saniabadi AR, Kuwano H, Nakano M.
Journal: Liver (2002): 321

Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method
Authors: Sakurai T, Terakawa S.
Journal: Methods Mol Biol (2002): 203

A HMG-CoA reductase inhibitor possesses a potent anti-atherosclerotic effect other than serum lipid lowering effects--the relevance of endothelial nitric oxide synthase and superoxide anion scavenging action
Authors: Sumi D, Hayashi T, Thakur NK, Jayachandran M, Asai Y, Kano H, Matsui H, Iguchi A.
Journal: Atherosclerosis (2001): 347

Continuous observation of superoxide generation in an in-situ ischemia-reperfusion rat lung model
Authors: Midorikawa J, Maehara K, Yaoita H, Watanabe T, Ohtani H, Ushiroda S, Maruyama Y.
Journal: Jpn Circ J (2001): 207

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