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MegaWox™ polyHRP-Protein A Conjugate

Ordering information
Price ()
Catalog Number11041
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Protein A is a 42-kDa protein found in the cell wall of Staphylococcus aureus. It binds the Fc region of immunoglobulins from various species with high affinity. There are four binding sites for antibodies but only two of them can be used simultaneously. Protein A conjugates are useful in the indirect detection of antigens immobilized on a solid support or in tissue samples. In these assays, an unlabeled detection antibody is added to the solid phase and then the immunocomplex formed is quantified with a protein A conjugate. Protein A conjugates may be used for the indirect detection of imunocomplexes in sandwich type immunoassays, provided that Fab fragments are used instead of whole antibody molecules for coating the solid phase. The affinity of protein A for immunoglobulins depends on the immunoglobulin class and the species. For example, the affinity is low for mouse IgG1 monoclonal antibodies and high for human and rabbit polyclonal antibodies. MegaWox™ polyHRP-Protein A Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The protein A-poly-HRP conjugate is purified to remove unconjugated protein A molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal.

References


View all 50 references: Citation Explorer
Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds.
Authors: Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: International journal of biological macromolecules (2020): 814-822
Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
Bioanalytical Application of Peroxidase-Mimicking DNAzymes: Status and Challenges.
Authors: Kosman, J and Juskowiak, B
Journal: Advances in biochemical engineering/biotechnology (2020): 59-84
A New Approach for Affinity-Based Purification of Horseradish Peroxidase.
Authors: Almaz, Zuleyha and Oztekin, Aykut and Abul, Nurgul and Gerni, Serpil and Erel, Deniz and Kocak, Seyma Mehtap and Sengül, Mehmet Emin and Ozdemir, Hasan
Journal: Biotechnology and applied biochemistry (2020)
New Reusable Solid Biosensor with Covalent Immobilization of the Horseradish Peroxidase Enzyme: In Situ Liberation Studies of Hydrogen Peroxide by Portable Chemiluminescent Determination.
Authors: Bocanegra-Rodríguez, Sara and Jornet-Martínez, Neus and Molins-Legua, Carmen and Campíns-Falcó, Pilar
Journal: ACS omega (2020): 2419-2427
A novel bioassay for the monitoring of hydrogen peroxide in human plasma samples based on binding of horseradish peroxidase-conjugated prostate specific antigen to poly (toluidine blue) as imprinted polymer receptor.
Authors: Sardaremelli, Sanam and Razmi, Habib and Hasanzadeh, Mohamad and Shadjou, Nasrin
Journal: International journal of biological macromolecules (2020): 311-324
Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme: Principle and Biosensing Application.
Authors: Alizadeh, Negar and Salimi, Abdollah and Hallaj, Rahman
Journal: Advances in biochemical engineering/biotechnology (2020): 85-106
Development of a new and simple method for the detection of histidine-tagged proteins based on thionine-chitosan/gold nanoparticles/horseradish peroxidase.
Authors: Ren, Ruijuan and Lu, Dingqiang and Pang, Guangchang
Journal: Biomedical microdevices (2020): 11
Development of a Surfactant-Containing Process to Improve the Removal Efficiency of Phenol and Control the Molecular Weight of Synthetic Phenolic Polymers Using Horseradish Peroxidase in an Aqueous System.
Authors: Yao, Chao-Ling and Lin, Che-Chi and Chu, I-Ming and Lai, Yi-Ting
Journal: Applied biochemistry and biotechnology (2020)
Amperometric Flow-Injection Analysis of Phenols Induced by Reactive Oxygen Species Generated under Daylight Irradiation of Titania Impregnated with Horseradish Peroxidase.
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Journal: Analytical chemistry (2020): 3643-3649