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MegaWox™ polyHRP-Streptavidin Conjugate

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Physical properties
Molecular weightN/A
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Molecular weight
Immunoassays (such as histochemistry, ELISAs and Western blotting) are important in clinical diagnosis and experimental protein analysis. These indirect analytical strategies primarily rely on the binding of an antigen by a specific antibody (primary antibody), followed by the detection of primary antibody by an enzyme or fluorescently labelled secondary antibody, allowing the semi-quantitative detection of a protein of interest. Histochemistry, ELISAs and Western blotting have been the common choices for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of these procedures. Among them, streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in a variety of immunoassays. The commercially available streptavidin and antibodies conjugated with many HRPs (PolyHRPs) have been widely used to enhance the detection sensitivity in immunoassays. MegaWox™ polyHRP-Streptavidin Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The streptavidin poly-HRP is purified to remove unconjugated streptavidin molecules that competes for binding sites with HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. MegaWox™ polyHRP-Streptavidin Conjugate is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays. Due to the extremely high sensitivity of MegaWox™ polyHRP-Streptavidin Conjugate a blocking procedure may been needed to eliminate the interference caused by the endogenous biotinylated proteins.


View all 10 references: Citation Explorer
One-pot microplate-based chemiluminescent assay coupled with catalytic hairpin assembly amplification for DNA detection.
Authors: Bodulev, Oleg L and Burkin, Konstantin M and Efremov, Eugene E and Sakharov, Ivan Yu
Journal: Analytical and bioanalytical chemistry (2020)
Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP).
Authors: Mishra, Manish and Tiwari, Shuchita and Gunaseelan, Anita and Li, Dongyang and Hammock, Bruce D and Gomes, Aldrin V
Journal: Electrophoresis (2019): 1731-1739
Development of a highly sensitive magneto-enzyme lateral flow immunoassay for dengue NS1 detection.
Authors: Tran, Tien V and Nguyen, Ba V and Nguyen, Thao T P and Tran, Tung T and Pham, Khanh G and Le, Quang B and Do, Binh N and Pham, Hung N and Nguyen, Chuyen V and Dinh, Duong P H and Ha, Van T and Doan, Trang H T and Le, Hoa Q
Journal: PeerJ (2019): e7779
Facilely prepared low-density DNA monolayer-based electrochemical biosensor with high detection performance in human serum.
Authors: Chen, Jinyuan and Ye, Chenliu and Liu, Zhoujie and Yang, Liangyong and Liu, Ailin and Zhong, Guangxian and Peng, Huaping and Lin, Xinhua
Journal: Analytical and bioanalytical chemistry (2019): 2101-2109
Nanobody Based Immunoassay for Human Soluble Epoxide Hydrolase Detection Using Polymeric Horseradish Peroxidase (PolyHRP) for Signal Enhancement: The Rediscovery of PolyHRP?
Authors: Li, Dongyang and Cui, Yongliang and Morisseau, Christophe and Gee, Shirley J and Bever, Candace S and Liu, Xiangjiang and Wu, Jian and Hammock, Bruce D and Ying, Yibin
Journal: Analytical chemistry (2017): 6248-6256
Automated 3D-printed unibody immunoarray for chemiluminescence detection of cancer biomarker proteins.
Authors: Tang, C K and Vaze, A and Rusling, J F
Journal: Lab on a chip (2017): 484-489
Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme.
Authors: Wen, Yanli and Wang, LeLe and Xu, Li and Li, Lanying and Ren, Suzhen and Cao, Chengming and Jia, Nengqin and Aldalbahi, Ali and Song, Shiping and Shi, Jiye and Xia, Jiaoyun and Liu, Gang and Zuo, Xiaolei
Journal: The Analyst (2016): 5304-10
Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay.
Authors: Szkola, A and Linares, E M and Worbs, S and Dorner, B G and Dietrich, R and Märtlbauer, E and Niessner, R and Seidel, M
Journal: The Analyst (2014): 5885-92
Plasma-derived mannose-binding lectin shows a direct interaction with C1-inhibitor.
Authors: Keizer, Mischa P and Kamp, Angela M and Brouwer, Nannette and van de Wetering, Marianne D and Wouters, Diana and Kuijpers, Taco W
Journal: Molecular immunology (2014): 187-93
Development and validation of an enzyme-linked immunosorbent assay for the quantification of trastuzumab in human serum and plasma.
Authors: Damen, Carola W N and de Groot, Els R and Heij, Marianne and Boss, David S and Schellens, Jan H M and Rosing, Hilde and Beijnen, Jos H and Aarden, Lucien A
Journal: Analytical biochemistry (2009): 114-20