MegaWox™ polyHRP-Streptavidin Conjugate

Immunoassays (such as histochemistry, ELISAs and Western blotting) are important in clinical diagnosis and experimental protein analysis. These indirect analytical strategies primarily rely on the binding of an antigen by a specific antibody (primary antibody), followed by the detection of primary antibody by an enzyme or fluorescently labelled secondary antibody, allowing the semi-quantitative detection of a protein of interest. Histochemistry, ELISAs and Western blotting have been the common choices for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of these procedures. Among them, streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in a variety of immunoassays. The commercially available streptavidin and antibodies conjugated with many HRPs (PolyHRPs) have been widely used to enhance the detection sensitivity in immunoassays. MegaWox™ polyHRP-Streptavidin Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The streptavidin poly-HRP is purified to remove unconjugated streptavidin molecules that competes for binding sites with HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. MegaWox™ polyHRP-Streptavidin Conjugate is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays. Due to the extremely high sensitivity of MegaWox™ polyHRP-Streptavidin Conjugate a blocking procedure may been needed to eliminate the interference caused by the endogenous biotinylated proteins.

General packaging for Cat. No. 11039. Your actual packaging may differ.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11039	1 mg	Price

References

View all 10 references: Citation Explorer

One-pot microplate-based chemiluminescent assay coupled with catalytic hairpin assembly amplification for DNA detection.

Authors:

Bodulev, Oleg L and Burkin, Konstantin M and Efremov, Eugene E and Sakharov, Ivan Yu

Journal:

Analytical and bioanalytical chemistry (2020)

Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP).

Authors:

Mishra, Manish and Tiwari, Shuchita and Gunaseelan, Anita and Li, Dongyang and Hammock, Bruce D and Gomes, Aldrin V

Journal:

Electrophoresis (2019): 1731-1739

Development of a highly sensitive magneto-enzyme lateral flow immunoassay for dengue NS1 detection.

Authors:

Tran, Tien V and Nguyen, Ba V and Nguyen, Thao T P and Tran, Tung T and Pham, Khanh G and Le, Quang B and Do, Binh N and Pham, Hung N and Nguyen, Chuyen V and Dinh, Duong P H and Ha, Van T and Doan, Trang H T and Le, Hoa Q

Journal:

PeerJ (2019): e7779

Facilely prepared low-density DNA monolayer-based electrochemical biosensor with high detection performance in human serum.

Authors:

Chen, Jinyuan and Ye, Chenliu and Liu, Zhoujie and Yang, Liangyong and Liu, Ailin and Zhong, Guangxian and Peng, Huaping and Lin, Xinhua

Journal:

Analytical and bioanalytical chemistry (2019): 2101-2109

Nanobody Based Immunoassay for Human Soluble Epoxide Hydrolase Detection Using Polymeric Horseradish Peroxidase (PolyHRP) for Signal Enhancement: The Rediscovery of PolyHRP?

Authors:

Li, Dongyang and Cui, Yongliang and Morisseau, Christophe and Gee, Shirley J and Bever, Candace S and Liu, Xiangjiang and Wu, Jian and Hammock, Bruce D and Ying, Yibin

Journal:

Analytical chemistry (2017): 6248-6256

Physical properties

Molecular weight	N/A
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025