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MegaWox™ polyHRP-Streptavidin Conjugate

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Price ()
Catalog Number11039
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Immunoassays (such as histochemistry, ELISAs and Western blotting) are important in clinical diagnosis and experimental protein analysis. These indirect analytical strategies primarily rely on the binding of an antigen by a specific antibody (primary antibody), followed by the detection of primary antibody by an enzyme or fluorescently labelled secondary antibody, allowing the semi-quantitative detection of a protein of interest. Histochemistry, ELISAs and Western blotting have been the common choices for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of these procedures. Among them, streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in a variety of immunoassays. The commercially available streptavidin and antibodies conjugated with many HRPs (PolyHRPs) have been widely used to enhance the detection sensitivity in immunoassays. MegaWox™ polyHRP-Streptavidin Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The streptavidin poly-HRP is purified to remove unconjugated streptavidin molecules that competes for binding sites with HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. MegaWox™ polyHRP-Streptavidin Conjugate is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays. Due to the extremely high sensitivity of MegaWox™ polyHRP-Streptavidin Conjugate a blocking procedure may been needed to eliminate the interference caused by the endogenous biotinylated proteins.

References


View all 10 references: Citation Explorer
One-pot microplate-based chemiluminescent assay coupled with catalytic hairpin assembly amplification for DNA detection.
Authors: Bodulev, Oleg L and Burkin, Konstantin M and Efremov, Eugene E and Sakharov, Ivan Yu
Journal: Analytical and bioanalytical chemistry (2020)
Facilely prepared low-density DNA monolayer-based electrochemical biosensor with high detection performance in human serum.
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Journal: Analytical and bioanalytical chemistry (2019): 2101-2109
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Journal: PeerJ (2019): e7779
Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP).
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Journal: Electrophoresis (2019): 1731-1739
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Journal: Lab on a chip (2017): 484-489
Nanobody Based Immunoassay for Human Soluble Epoxide Hydrolase Detection Using Polymeric Horseradish Peroxidase (PolyHRP) for Signal Enhancement: The Rediscovery of PolyHRP?
Authors: Li, Dongyang and Cui, Yongliang and Morisseau, Christophe and Gee, Shirley J and Bever, Candace S and Liu, Xiangjiang and Wu, Jian and Hammock, Bruce D and Ying, Yibin
Journal: Analytical chemistry (2017): 6248-6256
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