Name: MESG *Phosphate assay reagent*
Brand: AAT Bioquest
SKU: 21600
Availability: InStock

MESG

Phosphate assay reagent

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. This feature has been used to quantifying phosphate spectrophotometrically. We offer a convenient MESG-based phosphate assay kit (#21659).

Protocol

Catalog	Size	Price	Quantity
21600	5 mg	Price
21600-1MG	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MESG *Phosphate assay reagent* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	319.142 µL	1.596 mL	3.191 mL	15.957 mL	31.914 mL
5 mM	63.828 µL	319.142 µL	638.284 µL	3.191 mL	6.383 mL
10 mM	31.914 µL	159.571 µL	319.142 µL	1.596 mL	3.191 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	313.34 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Polyphosphate Coated Nanoparticles: Enzyme-Activated Charge-Reversal Gene Delivery Systems

Authors:

Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Ar{\i}soy, Sema and Friedl, Julian David and F{\"u}rst, Andrea and Saleh, Ahmad and Bernkop-Schn{\"u}rch, Andreas

Journal:

International Journal of Pharmaceutics (2023): 123474

Sacrificial crystal templating of hyaluronic acid-based hydrogels

Authors:

Thomas, Richelle C and Chung, Paul E and Modi, Shan P and Hardy, John G and Schmidt, Christine E

Journal:

European Polymer Journal (2016)

Osteogenic cell cultures cannot utilize exogenous sources of synthetic polyphosphate for mineralization

Authors:

Ariganello, Marianne B and Omelon, Sidney and Variola, Fabio and Wazen, Rima M and Moffatt, Pierre and Nanci, Antonio

Journal:

Journal of cellular biochemistry (2014): 2089--2102

References

View all 8 references: Citation Explorer

Relationship between activating and editing functions of the adenylation domain of apo-tyrocidin synthetase 1 (apo-TY1)

Authors:

Bucevic-Popovic V, Pavela-Vrancic M, Dieckmann R, Von Dohren H.

Journal:

Biochimie (2006): 265

Purine nucleoside phosphorylase activity in rat cerebrospinal fluid

Authors:

Silva RG, Santos DS, Basso LA, Oses JP, Wofchuk S, Portela LV, Souza DO.

Journal:

Neurochem Res (2004): 1831

Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors

Authors:

Tan YC, Wu H, Wang WN, Zheng Y, Wang ZX.

Journal:

Anal Biochem (2002): 156

A continuous spectrophotometric assay for aspartate transcarbamylase and ATPases

Authors:

Rieger CE, Lee J, Turnbull JL.

Journal:

Anal Biochem (1997): 86

Continuous monitoring of Pi release following nucleotide hydrolysis in actin or tubulin assembly using 2-amino-6-mercapto-7-methylpurine ribonucleoside and purine-nucleoside phosphorylase as an enzyme-linked assay

Authors:

Melki R, Fievez S, Carlier MF.

Journal:

Biochemistry (1996): 12038

Physical properties

Molecular weight	313.34
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	55727-10-1

Documents

Protocol
Safety Data Sheet (Catalog 21600)
Safety Data Sheet (Catalog 21600)
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits
Brochure: Physiological Probes & Assay Kits

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Page updated on November 3, 2025