Name: Metal Fluor™ Zn-520, AM
Brand: AAT Bioquest
SKU: 21263
Price: 232 USD
Availability: InStock

Metal Fluor™ Zn-520, AM

Metal Fluor™ Zn-520 is designed for detection of higher zinc ion concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists (0.05-50 µM) with minimal interfering calcium sensitivity. Metal Fluor™ Zn-520 has shown great fluorescence enhancement upon binding zinc ion (>250 folds). This cell permeant AM-ester is useful for detecting intracellular zinc ion levels.

Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Quantitation Kit. As low as 0.2 uM Zn2+ could be detected with 5 min incubation time.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21263	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Metal Fluor™ Zn-520, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	138.577 µL	692.885 µL	1.386 mL	6.929 mL	13.858 mL
5 mM	27.715 µL	138.577 µL	277.154 µL	1.386 mL	2.772 mL
10 mM	13.858 µL	69.289 µL	138.577 µL	692.885 µL	1.386 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	721.62 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

A Coordination Nanosystem Enables Endogenous Ferric Ion-Initiated Multi-Catalysis for Synergistic Tumor-Specific Ferroptosis and Gene Therapy

Authors:

Chen, Jiajie and Wang, Yitong and Yang, Zhibo and Tang, Kai and Liu, Xinchun and Ma, Hongshi and Ye, Huamao and Wu, Chengtie and Zhu, Yufang

Journal:

Small (2025): 2411440

Mitochondrial Dysfunction and Antioxidation Dyshomeostasis-Enhanced Tumor Starvation Synergistic Chemotherapy Achieved using a Metal--Organic Framework-Based Nano-Enzyme Reactor

Authors:

Huo, Taotao and Leilei chen, and Nie, Huifang and Li, Wenshuai and Lin, Chenteng and Akhtar, Muhammad and Huang, Rongqin

Journal:

ACS Applied Materials \& Interfaces (2022)

Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation

Authors:

Liu, Zixuan and Lv, Xuying and Xu, Lei and Liu, Xuting and Zhu, Xiangyu and Song, Erqun and Song, Yang

Journal:

Particle and fibre toxicology (2020): 1--17

References

View all 8 references: Citation Explorer

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment

Authors:

Colvin RA, Laskowski M, Fontaine CP.

Journal:

Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin

Authors:

Jansen S, Arning J, Dulcks T, Beyersmann D.

Journal:

Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes

Authors:

Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.

Journal:

J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments

Authors:

Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.

Journal:

Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester

Authors:

Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.

Journal:

J Org Chem (2000): 8204

Physical properties

Molecular weight	721.62
Solvent	DMSO

Spectral properties

Excitation (nm)	493
Emission (nm)	516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	FITC
Emission	FITC
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

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Shipping	Standard overnight for United States, inquire for international

Page updated on October 10, 2025