Metal Fluor™ Zn-520, AM
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 721.62 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 516 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Metal Fluor™ Zn-520, Potassium Salt |
Overview | SDSProtocol |
See also: Intracellular Ions, Intracellular Zinc
Molecular weight 721.62 | Excitation (nm) 493 | Emission (nm) 516 |
Metal Fluor™ Zn-520 is designed for detection of higher zinc ion concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists (0.05-50 µM) with minimal interfering calcium sensitivity. Metal Fluor™ Zn-520 has shown great fluorescence enhancement upon binding zinc ion (>250 folds). This cell permeant AM-ester is useful for detecting intracellular zinc ion levels.
Platform
Fluorescence microscope
Excitation | FITC |
Emission | FITC |
Recommended plate | Black wall/clear bottom |
Fluorescence microplate reader
Excitation | 490 |
Emission | 525 |
Cutoff | 515 |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Bottom read mode |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Metal Fluor™ Zn-520, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 138.577 µL | 692.885 µL | 1.386 mL | 6.929 mL | 13.858 mL |
5 mM | 27.715 µL | 138.577 µL | 277.154 µL | 1.386 mL | 2.772 mL |
10 mM | 13.858 µL | 69.289 µL | 138.577 µL | 692.885 µL | 1.386 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Citations
View all 2 citations: Citation Explorer
Mitochondrial Dysfunction and Antioxidation Dyshomeostasis-Enhanced Tumor Starvation Synergistic Chemotherapy Achieved using a Metal--Organic Framework-Based Nano-Enzyme Reactor
Authors: Huo, Taotao and Leilei chen, and Nie, Huifang and Li, Wenshuai and Lin, Chenteng and Akhtar, Muhammad and Huang, Rongqin
Journal: ACS Applied Materials \& Interfaces (2022)
Authors: Huo, Taotao and Leilei chen, and Nie, Huifang and Li, Wenshuai and Lin, Chenteng and Akhtar, Muhammad and Huang, Rongqin
Journal: ACS Applied Materials \& Interfaces (2022)
Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation
Authors: Liu, Zixuan and Lv, Xuying and Xu, Lei and Liu, Xuting and Zhu, Xiangyu and Song, Erqun and Song, Yang
Journal: Particle and fibre toxicology (2020): 1--17
Authors: Liu, Zixuan and Lv, Xuying and Xu, Lei and Liu, Xuting and Zhu, Xiangyu and Song, Erqun and Song, Yang
Journal: Particle and fibre toxicology (2020): 1--17
References
View all 8 references: Citation Explorer
Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1
S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145
Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889
Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538
The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204
Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097
Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781
Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
FAQ
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can you provide a brief protocol on how to use coelenterazine h for small animal in vivo imaging?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can you provide a brief protocol on how to use coelenterazine h for small animal in vivo imaging?