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Metal Fluor™ Zn-520, AM

Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Quantitation Kit. As low as 0.2 uM Zn2+ could be detected with 5 min incubation time.
Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Quantitation Kit. As low as 0.2 uM Zn2+ could be detected with 5 min incubation time.
Zinc Chloride dose response was measured on a 96-well black plate with the Amplite® Fluorimetric Zinc Quantitation Kit. As low as 0.2 uM Zn2+ could be detected with 5 min incubation time.
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Physical properties
Molecular weight721.62
SolventDMSO
Spectral properties
Excitation (nm)493
Emission (nm)516
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
721.62
Excitation (nm)
493
Emission (nm)
516
Metal Fluor™ Zn-520 is designed for detection of higher zinc ion concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists (0.05-50 µM) with minimal interfering calcium sensitivity. Metal Fluor™ Zn-520 has shown great fluorescence enhancement upon binding zinc ion (>250 folds). This cell permeant AM-ester is useful for detecting intracellular zinc ion levels.

Platform


Fluorescence microscope

ExcitationFITC
EmissionFITC
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader

Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Metal Fluor™ Zn-520, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM138.577 µL692.885 µL1.386 mL6.929 mL13.858 mL
5 mM27.715 µL138.577 µL277.154 µL1.386 mL2.772 mL
10 mM13.858 µL69.289 µL138.577 µL692.885 µL1.386 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)493
Emission (nm)516

Images


Citations


View all 2 citations: Citation Explorer
Mitochondrial Dysfunction and Antioxidation Dyshomeostasis-Enhanced Tumor Starvation Synergistic Chemotherapy Achieved using a Metal--Organic Framework-Based Nano-Enzyme Reactor
Authors: Huo, Taotao and Leilei chen, and Nie, Huifang and Li, Wenshuai and Lin, Chenteng and Akhtar, Muhammad and Huang, Rongqin
Journal: ACS Applied Materials \& Interfaces (2022)
Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation
Authors: Liu, Zixuan and Lv, Xuying and Xu, Lei and Liu, Xuting and Zhu, Xiangyu and Song, Erqun and Song, Yang
Journal: Particle and fibre toxicology (2020): 1--17

References


View all 8 references: Citation Explorer
Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1
S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145
Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889
Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538
The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204
Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097
Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781
Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153