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Methylene Blue Alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 492.52 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 662 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Methylene Blue Maleimide |
| Methylene Blue Azide |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 492.52 | Absorbance (nm) 662 |
Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue Alkyne is an excellent building block for preparing MB-labeled nucleic acid probes from azido-modified oligonucleotides, including picolyl azide-modified oligonucleotides via the well-known click chemistry. MB is also a well-known photosensitizer used in photodynamic therapy (PDT) and investigated for the topical treatment of various skin infections via PDT modality. It is a common biological stain, commonly used in medical laboratories to stain cells and tissues for microscopic examination. It can help visualize cellular structures and identify abnormalities. In addition, MB has been studied for its potential role in treating neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Some research suggests it might have a protective effect on neurons and mitochondrial function.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Methylene Blue Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 203.037 µL | 1.015 mL | 2.03 mL | 10.152 mL | 20.304 mL |
| 5 mM | 40.607 µL | 203.037 µL | 406.075 µL | 2.03 mL | 4.061 mL |
| 10 mM | 20.304 µL | 101.519 µL | 203.037 µL | 1.015 mL | 2.03 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue Alkyne is an excellent building block for preparing MB-labeled nucleic acid probes from azido-modified oligonucleotides, including picolyl azide-modified oligonucleotides via the well-known click chemistry.
References
View all 50 references: Citation Explorer
[Retrospective analysis of BI-RADS 4 and 5 lesions additionally detected by breast MRI].
Authors: Jiang, L and Gao, J Y and Xu, Z J and He, S R and Hua, B
Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (2023): 100-106
Authors: Jiang, L and Gao, J Y and Xu, Z J and He, S R and Hua, B
Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (2023): 100-106
Preoperative localization of sentinel lymph nodes using percutaneous contrast-enhanced ultrasonography in patients with breast cancer.
Authors: Xu, Yi-Lin and Liu, Xue-Jing and Zhu, Ying and Lu, Hong
Journal: Gland surgery (2022): 369-377
Authors: Xu, Yi-Lin and Liu, Xue-Jing and Zhu, Ying and Lu, Hong
Journal: Gland surgery (2022): 369-377
Pseudoreticulocytosis by the ADVIA 2120 Hematology Analyzer and Other Hematologic Changes in a Cynomolgus Macaque (Macaca fascicularis) With Malaria.
Authors: Sharma, Diya and Priest, Heather and Wilcox, Angela
Journal: Toxicologic pathology (2022): 684-692
Authors: Sharma, Diya and Priest, Heather and Wilcox, Angela
Journal: Toxicologic pathology (2022): 684-692
Dataset of multiple methodology characterization of an illite-kaolinite clay mineral for the purpose of using it as ceramic membrane supports.
Authors: Elgamouz, Abdelaziz and Tijani, Najib and Shehadi, Ihsan and Hasan, Kamrul and Al-Farooq Kawam, Mohamad
Journal: Data in brief (2020): 105300
Authors: Elgamouz, Abdelaziz and Tijani, Najib and Shehadi, Ihsan and Hasan, Kamrul and Al-Farooq Kawam, Mohamad
Journal: Data in brief (2020): 105300
Disposal of the large volume of sputum positive for Mycobacterium tuberculosis by using microwave sterilisation technology as an alternative to traditional autoclaving in a tertiary respiratory care hospital in Delhi, India.
Authors: Myneedu, Vithal Prasad and Aggarwal, Amit
Journal: Infection prevention in practice (2020): 100072
Authors: Myneedu, Vithal Prasad and Aggarwal, Amit
Journal: Infection prevention in practice (2020): 100072
Characterization of the firing behaviour of an illite-kaolinite clay mineral and its potential use as membrane support.
Authors: Elgamouz, Abdelaziz and Tijani, Najib and Shehadi, Ihsan and Hasan, Kamrul and Al-Farooq Kawam, Mohamad
Journal: Heliyon (2019): e02281
Authors: Elgamouz, Abdelaziz and Tijani, Najib and Shehadi, Ihsan and Hasan, Kamrul and Al-Farooq Kawam, Mohamad
Journal: Heliyon (2019): e02281
A Quicker Tzanck Smear with Methylene Blue Stain for Diagnosis of Herpesvirus Skin Infections: a Comparative Study of Giemsa Stain.
Authors: Wantavornprasert, Kittipong and Chottawornsak, Natcha and Hurst, Cameron Paul and Asawanonda, Pravit and Hansasuta, Pokrath and Rerknimitr, Pawinee
Journal: Japanese journal of infectious diseases (2019): 432-434
Authors: Wantavornprasert, Kittipong and Chottawornsak, Natcha and Hurst, Cameron Paul and Asawanonda, Pravit and Hansasuta, Pokrath and Rerknimitr, Pawinee
Journal: Japanese journal of infectious diseases (2019): 432-434
Benjamin Terry and his rapid razor section method for intraoperative diagnosis.
Authors: Duchesne, Stéphan R and Naugler, Christopher and Wright, James R
Journal: Journal of medical biography (2018): 156-164
Authors: Duchesne, Stéphan R and Naugler, Christopher and Wright, James R
Journal: Journal of medical biography (2018): 156-164
[Mycoplasma genitalium infection in patients attending the STD clinic in Nanjing].
Authors: Wang, Bi-Wei and Li, Sai and Su, Xiao-Hong and LE, Wen-Jing and Zhang, Jin-Ping and Sha, Zhong and You, Yong-Yan
Journal: Zhonghua nan ke xue = National journal of andrology (2018): 1073-1077
Authors: Wang, Bi-Wei and Li, Sai and Su, Xiao-Hong and LE, Wen-Jing and Zhang, Jin-Ping and Sha, Zhong and You, Yong-Yan
Journal: Zhonghua nan ke xue = National journal of andrology (2018): 1073-1077
Biosynthesis of nano cupric oxide on cotton using Seidlitzia rosmarinus ashes utilizing bio, photo, acid sensing and leaching properties.
Authors: Bashiri Rezaie, Ali and Montazer, Majid and Rad, Mahnaz Mahmoudi
Journal: Carbohydrate polymers (2017): 1-12
Authors: Bashiri Rezaie, Ali and Montazer, Majid and Rad, Mahnaz Mahmoudi
Journal: Carbohydrate polymers (2017): 1-12