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Methylene Blue Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 523.54 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 662 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Methylene Blue Maleimide |
| Methylene Blue Alkyne |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 523.54 | Absorbance (nm) 662 |
Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue Azide is an excellent building block for preparing MB-labeled nucleic acid probes from alkyne-modified oligonucleotides, including DBCO-modified oligonucleotides. MB is also a well-known photosensitizer used in photodynamic therapy (PDT) and investigated for the topical treatment of various skin infections via PDT modality. It is a common biological stain, commonly used in medical laboratories to stain cells and tissues for microscopic examination. It can help visualize cellular structures and identify abnormalities. In addition, MB has been studied for its potential role in treating neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Some research suggests it might have a protective effect on neurons and mitochondrial function.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Methylene Blue Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 191.007 µL | 955.037 µL | 1.91 mL | 9.55 mL | 19.101 mL |
| 5 mM | 38.201 µL | 191.007 µL | 382.015 µL | 1.91 mL | 3.82 mL |
| 10 mM | 19.101 µL | 95.504 µL | 191.007 µL | 955.037 µL | 1.91 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Methylene Blue (MB) is widely used as a redox indicator in nucleic acid research. Methylene Blue Azide is an excellent building block for preparing MB-labeled nucleic acid probes from alkyne-modified oligonucleotides, including DBCO-modified oligonucleotides.
References
View all 46 references: Citation Explorer
Hybrid ceramic repair strength, surface roughness, and bond failure, using methylene blue-activated low-level laser therapy, Carbon dioxide, and Ti: Al2O3 laser.
Authors: Alsunbul, Hanan and Almutairi, Basil and Aljanakh, Mohammad and Abduljabbar, Tariq
Journal: Photodiagnosis and photodynamic therapy (2023): 103693
Authors: Alsunbul, Hanan and Almutairi, Basil and Aljanakh, Mohammad and Abduljabbar, Tariq
Journal: Photodiagnosis and photodynamic therapy (2023): 103693
Incorporation of Zirconium Oxide Nanoparticles in Adhesive Resin for Bonding of Brackets to Enamel Conditioned with Er,Cr:YSGG, Photodynamic Therapy, and Phosphoric Acid.
Authors: Alqerban, Ali and Asiri, Saeed N and Alharbi, Fahad and Alqhtani, Nasser and Alenazi, Adel and Robaian, Ali and Samran, Abdulaziz
Journal: Photobiomodulation, photomedicine, and laser surgery (2023): 218-224
Authors: Alqerban, Ali and Asiri, Saeed N and Alharbi, Fahad and Alqhtani, Nasser and Alenazi, Adel and Robaian, Ali and Samran, Abdulaziz
Journal: Photobiomodulation, photomedicine, and laser surgery (2023): 218-224
Radicular canal disinfection by photosensitizers activated by photodynamic therapy and Er, Cr: YSGG laser bonded to glass fiber post using different cement types. An Invitro study.
Authors: Qamar, Zeeshan and Niazi, Fayez Hussain and Alshalan, Abdulrahman Mohammed and Almutairi, Waleed Tayi and Lingam, Amara Swapna
Journal: Photodiagnosis and photodynamic therapy (2022): 102730
Authors: Qamar, Zeeshan and Niazi, Fayez Hussain and Alshalan, Abdulrahman Mohammed and Almutairi, Waleed Tayi and Lingam, Amara Swapna
Journal: Photodiagnosis and photodynamic therapy (2022): 102730
Effects of methylene blue and curcumin photosensitizers on the color stability of endodontically treated intraradicular dentin.
Authors: Sivieri-Araujo, Gustavo and Strazzi-Sahyon, Henrico Badaoui and Jacomassi, Denis Pablo and Dos Santos, Paulo Henrique and Cintra, Luciano Tavares Angelo and Kurachi, Cristina and Bagnato, Vanderlei Salvador
Journal: Photodiagnosis and photodynamic therapy (2022): 102650
Authors: Sivieri-Araujo, Gustavo and Strazzi-Sahyon, Henrico Badaoui and Jacomassi, Denis Pablo and Dos Santos, Paulo Henrique and Cintra, Luciano Tavares Angelo and Kurachi, Cristina and Bagnato, Vanderlei Salvador
Journal: Photodiagnosis and photodynamic therapy (2022): 102650
Natural and synthetic photosensitizers activated by photodynamic therapy on enamel reconditioning rebonded to metallic brackets: an in vitro study.
Authors: Kamran, M A and Almoammar, S and Alnazeh, A A and Alshahrani, A and Alhaizaey, A H A and Alshahrani, I
Journal: European review for medical and pharmacological sciences (2022): 9089-9097
Authors: Kamran, M A and Almoammar, S and Alnazeh, A A and Alshahrani, A and Alhaizaey, A H A and Alshahrani, I
Journal: European review for medical and pharmacological sciences (2022): 9089-9097
Effect of low-power diode laser on infected root canals.
Authors: Alves, Denise Ramos Silveira and Decucio, Daniel de Almeida and Alencar, Ana Helena Gonçalves de and Estrela, Cyntia Rodrigues de Araújo and Souza, João Batista de and Pinheiro, Antônio Luiz Barbosa and Estrela, Carlos
Journal: Brazilian dental journal (2022): 8-17
Authors: Alves, Denise Ramos Silveira and Decucio, Daniel de Almeida and Alencar, Ana Helena Gonçalves de and Estrela, Cyntia Rodrigues de Araújo and Souza, João Batista de and Pinheiro, Antônio Luiz Barbosa and Estrela, Carlos
Journal: Brazilian dental journal (2022): 8-17
Advanced methylene blue - nanoemulsions for in vitro photodynamic therapy on oral and cervical human carcinoma.
Authors: do Amaral, Stéphanie R and Amantino, Camila F and De Annunzio, Sarah R and de Paula, Ariela V and Fontana, Carla R and Primo, Fernando L
Journal: Lasers in medical science (2022): 3443-3450
Authors: do Amaral, Stéphanie R and Amantino, Camila F and De Annunzio, Sarah R and de Paula, Ariela V and Fontana, Carla R and Primo, Fernando L
Journal: Lasers in medical science (2022): 3443-3450
Efficacy of antimicrobial photodynamic therapy on the tongue surface in the management of halitosis - A real-time polymerase chain reaction analysis.
Authors: Patil, Pooja and Patil, Leena and Triveni, M G and Usha, G V and Shah, Rucha and Kumar, A B Tarun
Journal: Photodiagnosis and photodynamic therapy (2022): 102989
Authors: Patil, Pooja and Patil, Leena and Triveni, M G and Usha, G V and Shah, Rucha and Kumar, A B Tarun
Journal: Photodiagnosis and photodynamic therapy (2022): 102989
In vitro evaluation of EDTA combined with photodynamic therapy to reduce Streptococcus mutans in carious dentin.
Authors: Fernandes, Fernanda Geovana Lopes and de Moraes, Franciele Bartolomeu and De Cezare, Juliana Ando and Degasperi, Giovanna Rosa and Fontana, Carlos Eduardo and Grandizoli, Diana Roberta Pereira and Pinheiro, Sérgio Luiz
Journal: Photodiagnosis and photodynamic therapy (2022): 102718
Authors: Fernandes, Fernanda Geovana Lopes and de Moraes, Franciele Bartolomeu and De Cezare, Juliana Ando and Degasperi, Giovanna Rosa and Fontana, Carlos Eduardo and Grandizoli, Diana Roberta Pereira and Pinheiro, Sérgio Luiz
Journal: Photodiagnosis and photodynamic therapy (2022): 102718
Influence of antimicrobial photodynamic therapy with different pre-irradiation times on children's dental biofilm: randomized clinical trial.
Authors: Lima, N G and Monteiro, R M and Torres, C P and de Souza-Gabriel, A E and Watanabe, E and Borsatto, M C
Journal: European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry (2022): 897-904
Authors: Lima, N G and Monteiro, R M and Torres, C P and de Souza-Gabriel, A E and Watanabe, E and Borsatto, M C
Journal: European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry (2022): 897-904