Name: mFluor™ Blue 570 acid
Brand: AAT Bioquest
SKU: 1143
Price: 348 USD
Availability: InStock

mFluor™ Blue 570 acid

mFluor™ Blue 570 dyes are an excellent alternative to RPE since they have the spectral properties equivalent to those of RPE conjugates. mFluor™ Blue 570 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Blue 570 dyes are well excited at 488 nm to give red fluorescence (compatible with TRITC filter). mFluor™ Blue 570 dye and conjugates are excellent blue laser reagents for flow cytometry research. Compared to RPE, mFluor™ Blue 570 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates.

<p>With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1143	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Blue 570 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	69.766 µL	348.828 µL	697.657 µL	3.488 mL	6.977 mL
5 mM	13.953 µL	69.766 µL	139.531 µL	697.657 µL	1.395 mL
10 mM	6.977 µL	34.883 µL	69.766 µL	348.828 µL	697.657 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1433.37 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1433.37
Solvent	DMSO

Spectral properties

Absorbance (nm)	553
Correction factor (260 nm)	0.228
Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	505
Emission (nm)	564

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 28, 2025