Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

mFluor™ Blue 570 acid

With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Ordering information
Price ()
Catalog Number1143
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1433.37
SolventDMSO
Spectral properties
Absorbance (nm)553
Correction Factor (260 nm)0.228
Correction Factor (280 nm)0.179
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)505
Emission (nm)564
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
mFluor™ Violet 450 SE
mFluor™ Violet 510 SE
mFluor™ Violet 540 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE
mFluor™ Red 700 SE
mFluor™ Red 780 SE
mFluor™ Red 780 amine
mFluor™ Violet 450-VAD-FMK
mFluor™ 510-VAD-FMK
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
mFluor™ Violet 450 acid
mFluor™ Violet 510 acid
mFluor™ Violet 540 acid
mFluor™ Green 620 acid
mFluor™ Yellow 630 acid
mFluor™ Red 700 acid
mFluor™ Violet 500 SE
mFluor™ UV375 SE
mFluor™ Red 780 acid
mFluor™ UV460 SE
mFluor™ Blue 630 SE
mFluor™ Blue 660 SE
mFluor™ Violet 610 SE
mFluor™ Green 630 SE
mFluor™ Blue 580 SE
mFluor™ Blue 590 SE
mFluor™ Blue 620 SE
mFluor™ Red 780 Maleimide
mFluor™ Violet 550 SE
mFluor™ Violet 505 SE
mFluor™ Violet 590 SE
mFluor™ Violet 545 SE
mFluor™ UV420 SE
mFluor™ UV455 SE
mFluor™ UV520 SE
mFluor™ UV540 SE
mFluor™ UV610 SE
mFluor™ Violet 450 Azide
mFluor™ Violet 450 maleimide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
mFluor™ Violet 530 SE
mFluor™ Violet 530 maleimide
Show More (55)

OverviewpdfSDSpdfProtocol


Molecular weight
1433.37
Absorbance (nm)
553
Correction Factor (260 nm)
0.228
Correction Factor (280 nm)
0.179
Extinction coefficient (cm -1 M -1)
1200001
Excitation (nm)
505
Emission (nm)
564
mFluor™ Blue 570 dyes are an excellent alternative to RPE since they have the spectral properties equivalent to those of RPE conjugates. mFluor™ Blue 570 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Blue 570 dyes are well excited at 488 nm to give red fluorescence (compatible with TRITC filter). mFluor™ Blue 570 dye and conjugates are excellent blue laser reagents for flow cytometry research. Compared to RPE, mFluor™ Blue 570 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Blue 570 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM69.766 µL348.828 µL697.657 µL3.488 mL6.977 mL
5 mM13.953 µL69.766 µL139.531 µL697.657 µL1.395 mL
10 mM6.977 µL34.883 µL69.766 µL348.828 µL697.657 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)553
Correction Factor (260 nm)0.228
Correction Factor (280 nm)0.179
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)505
Emission (nm)564

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
mFluor™ Blue 570 SE55356512000010.2280.179

Citations


View all 3 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References


View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: J Neurochem (2010): 994
Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint "Chengshan" (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506