Name: mFluor™ Blue 570 SE
Brand: AAT Bioquest
SKU: 1160
Price: 355 USD
Availability: InStock

mFluor™ Blue 570 SE

mFluor™ Blue 570 SE is a water-soluble, amine-reactive fluorescent dye for labeling proteins, antibodies, peptides, and amino-modified oligonucleotides for flow cytometry and fluorescence imaging applications.

Spectral Properties: Excitation at 505 nm and emission at 564 nm.
Photostability: Greater resistance to photobleaching than RPE.
Reaction Chemistry: NHS ester reacts with primary amines to form stable conjugates.
Instrument Compatibility: Protein conjugates support 488 nm blue-laser excitation and TRITC filters.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1160	1 mg	Price

Overview

mFluor™ Blue 570 dyes are an excellent alternative to RPE since they have the spectral properties equivalent to those of RPE conjugates. mFluor™ Blue 570 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Blue 570 dyes are well excited at 488 nm to give red fluorescence (compatible with TRITC filter). mFluor™ Blue 570 dye and conjugates are excellent blue laser reagents for flow cytometry research. Compared to RPE, mFluor™ Blue 570 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Blue 570 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	54.518 µL	272.591 µL	545.182 µL	2.726 mL	5.452 mL
5 mM	10.904 µL	54.518 µL	109.036 µL	545.182 µL	1.09 mL
10 mM	5.452 µL	27.259 µL	54.518 µL	272.591 µL	545.182 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1834.25 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Ex vivo single-cell profiling of acute myocardial infarction patients reveals disproportionate CD66b+ cell secretion response

Authors:

Zeming, Kerwin Kwek and Lu, Ri and Lee, Elizabeth and Cheung, Ka-Wai and Chew, Nicholas WS and Woo, Kai Lee and Cheow, Lih Feng and Han, Jongyoon and Lim, Shir Lynn

Journal:

Bioengineering \& Translational Medicine (2025): e70043

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra and Martens, Erik and Liekens, S and ra and Noppen, Sam and Damme, Jo Van and others

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1834.25
Solvent	DMSO

Spectral properties

Absorbance (nm)	553
Correction factor (260 nm)	0.228
Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	505
Emission (nm)	564

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 10, 2026