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mFluor™ Red 700 acid

With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Ordering information
Price ()
Catalog Number1146
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1994.41
SolventDMSO
Spectral properties
Absorbance (nm)651
Correction Factor (260 nm)0.135
Correction Factor (280 nm)0.127
Extinction coefficient (cm -1 M -1)2950001
Excitation (nm)680
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1994.41
Absorbance (nm)
651
Correction Factor (260 nm)
0.135
Correction Factor (280 nm)
0.127
Extinction coefficient (cm -1 M -1)
2950001
Excitation (nm)
680
Emission (nm)
695
mFluor™ Red 700 dyes are an excellent alternative to APC-Alexa Fluor® 700 tandems since they have the spectral properties equivalent to those of APC-Alexa Fluor® 700 conjugates. mFluor™ Red 700 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Red 700 dyes are well excited at 633 nm to give red fluorescence (compatible with Cy5.5® filter). mFluor™ Red 700 dyes and conjugates are excellent red laser reagents for flow cytometry research. Compared to APC-Alexa Fluor® 700 dyes, mFluor™ Red 700 dyes are much more photostable than the spectrally similar APC tandems, making them readily available for fluorescence imaging applications while it is very difficult to use a APC-Alexa Fluor® 700 conjugates for fluorescence imaging applications due to the rapid photobleaching of the APC tandems (such as APC-Alexa Fluor® 700).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Red 700 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM50.14 µL250.701 µL501.401 µL2.507 mL5.014 mL
5 mM10.028 µL50.14 µL100.28 µL501.401 µL1.003 mL
10 mM5.014 µL25.07 µL50.14 µL250.701 µL501.401 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)651
Correction Factor (260 nm)0.135
Correction Factor (280 nm)0.127
Extinction coefficient (cm -1 M -1)2950001
Excitation (nm)680
Emission (nm)695

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
mFluor™ Red 700 SE68069529500010.1350.127
mFluor™ Red 780 acid6297679000010.1010.116

Citations


View all 3 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References


View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
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Journal: J Neurochem (2010): 994
Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
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Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint "Chengshan" (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506