mFluor™ Red 700 SE

mFluor™ Red 700 dyes are an excellent alternative to APC-Alexa Fluor® 700 tandems since they have the spectral properties equivalent to those of APC-Alexa Fluor® 700 conjugates. mFluor™ Red 700 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Red 700 dyes are well excited at 633 nm to give red fluorescence (compatible with Cy5.5® filter). mFluor™ Red 700 dyes and conjugates are excellent red laser reagents for flow cytometry research. Compared to APC-Alexa Fluor® 700 dyes, mFluor™ Red 700 dyes are much more photostable than the spectrally similar APC tandems, making them readily available for fluorescence imaging applications while it is very difficult to use a APC-Alexa Fluor® 700 conjugates for fluorescence imaging applications due to the rapid photobleaching of the APC tandems (such as APC-Alexa Fluor® 700).

<p>Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by mFluor™Red 700 conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.</p>

Catalog	Size	Price	Quantity
1190	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Red 700 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.475 µL	522.373 µL	1.045 mL	5.224 mL	10.447 mL
5 mM	20.895 µL	104.475 µL	208.949 µL	1.045 mL	2.089 mL
10 mM	10.447 µL	52.237 µL	104.475 µL	522.373 µL	1.045 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	957.17 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	957.17
Solvent	DMSO

Spectral properties

Absorbance (nm)	651
Correction factor (260 nm)	0.135
Correction factor (280 nm)	0.127
Extinction coefficient (cm ^-1 M ^-1)	295000 ¹
Excitation (nm)	680
Emission (nm)	695

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on July 26, 2023