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mFluor™ Red 700-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluor™ Red 700-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ Red 700 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 633 nm excitation (e.g., the Red Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
<p>Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by mFluor&trade;Red 700 conjugate (Cat#16946). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.</p>
<p>Flow cytometry analysis of HL-60 cells stained with (Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by mFluor&trade;Red 700 conjugate (Cat#16946). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16946100 ug
Price
 
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Absorbance (nm)651
Correction factor (260 nm)0.135
Correction factor (280 nm)0.127
Extinction coefficient (cm -1 M -1)
295000
1
Excitation (nm)680
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 3, 2025