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mFluor™ UV 375 Biotin Conjugate

mFluor™ UV 375 Biotin Conjugate contains mFluor™ UV 375 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong UV fluorescence.
mFluor™ UV 375 Biotin Conjugate contains mFluor™ UV 375 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong UV fluorescence.
Ordering information
Price ()
Catalog Number3111
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight954.26
SolventDMSO
Spectral properties
Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
954.26
Absorbance (nm)
342
Correction Factor (260 nm)
0.099
Correction Factor (280 nm)
0.138
Extinction coefficient (cm -1 M -1)
300001
Excitation (nm)
351
Emission (nm)
387
Quantum yield
0.941
mFluor™ UV 375 Biotin Conjugate contains mFluor™ UV 375 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong UV fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ UV 375 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UV 375 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM104.793 µL523.966 µL1.048 mL5.24 mL10.479 mL
5 mM20.959 µL104.793 µL209.586 µL1.048 mL2.096 mL
10 mM10.479 µL52.397 µL104.793 µL523.966 µL1.048 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 460 Biotin Conjugate3584561500010.8610.350.134

References


View all 47 references: Citation Explorer
An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates.
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots.
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Integration of Enzymatic Labeling with Single-Molecule Detection for Sensitive Quantification of Diverse DNA Damages.
Authors: Zhang, Yan and Hua, Ruo-Nan and Zhang, Chun-Yang
Journal: Analytical chemistry (2020): 4700-4706
A Vector-Based Method to Analyze the Topography of Glial Networks.
Authors: Eitelmann, Sara and Hirtz, Jan J and Stephan, Jonathan
Journal: International journal of molecular sciences (2019)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Enhanced broadband fluorescence detection of nucleic acids using multipolar gap-plasmons on biomimetic Au metasurfaces.
Authors: Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck
Journal: Nanoscale (2019): 13750-13757
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74