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mFluor™ UV375 SE
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 355 nm, 405 nm, 488 nm and 633 nm). mFluor™ UV375 (MFUV375) dyes have fluorescence excitation and emission maxima of ~355 nm and ~375 nm respectively. These spectral characteristics make them an excellent alternative to BD Biosciences' BUV395. mFluor™ UV375 dyes are well excited by UV laser of 355 nm with emission centered around 375 nm, which perfectly matches the filter set of 379/28 nm used for BUV 395. In contrast to polymer-based BUV 395 dye, MFUV375 is a small organic molecule that can be readily conjugated to antibodies. It is significantly dimmer than BUV395, might be used for brighter markers (such as CD4).
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with mFluor&trade; UV375 Anti-human CD4 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; UV375 specific UV2-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with mFluor&trade; UV375 Anti-human CD4 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; UV375 specific UV2-A channel.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
11351 mg
Price
 
Physical properties
Molecular weight782.95
SolventDMSO
Spectral properties
Absorbance (nm)342
Correction factor (260 nm)0.099
Correction factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)
30000
1
Excitation (nm)351
Emission (nm)387
Quantum yield
0.94
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 4, 2025