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mFluor™ UV540 maleimide
mFluor™ dyes are engineered for advanced multicolor flow cytometry applications, offering significant Stokes Shifts and optimal excitation by standard flow cytometer laser lines, such as 350 nm, 405 nm, 488 nm, 532 nm, and 633 nm. The mFluor™ UV540 dye, which exhibits a bright yellow fluorescence and moderate photostability, is ideally suited for excitation at both the 350 nm and 532 nm laser lines, with an emission maximum at ~560 nm. This maleimide derivative is commonly used for the conjugation of thiol groups on proteins, oligonucleotide thiophosphates, and low molecular weight ligands, producing conjugates that exhibit significantly brighter fluorescence and enhanced photostability compared to those formed with other spectrally similar fluorophores.
<b>Top)</b> The spectral pattern was obtained using a 4-laser spectral cytometer. Lasers at wavelengths of 355 nm, 405 nm, 488 nm, and 640 nm were spatially offset to generate four distinct emission profiles. The combination of these profiles resulted in the overall spectral signature. <b>Bottom)</b> Flow cytometry analysis was conducted on whole blood cells stained with the CD4-mFluor™ UV540 conjugate. The fluorescence signal was detected using an Aurora spectral flow cytometer, specifically in the UV9-A channel optimized for mFluor™ UV540.
<b>Top)</b> The spectral pattern was obtained using a 4-laser spectral cytometer. Lasers at wavelengths of 355 nm, 405 nm, 488 nm, and 640 nm were spatially offset to generate four distinct emission profiles. The combination of these profiles resulted in the overall spectral signature. <b>Bottom)</b> Flow cytometry analysis was conducted on whole blood cells stained with the CD4-mFluor™ UV540 conjugate. The fluorescence signal was detected using an Aurora spectral flow cytometer, specifically in the UV9-A channel optimized for mFluor™ UV540.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
16081 mg
Price
 
Physical properties
Molecular weight1232.26
SolventDMSO
Spectral properties
Absorbance (nm)541
Correction factor (260 nm)0.634
Correction factor (280 nm)0.463
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)368
Emission (nm)560
Quantum yield
0.35
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Flow Cytometry Fluorochromes
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Page updated on December 14, 2025