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mFluor™ UV540 SE
mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ UV dyes are optimized to be excited with a UV laser at 350 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by UV laser at 350 nm. mFluor™ UV 540 dyes have fluorescence excitation and emission maxima of ~350 nm and ~540 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ UV 540 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ UV 540 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the UV laser excitation.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; UV540 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; UV540 specific UV9-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; UV540 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; UV540 specific UV9-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16451 mg
Price
 
Physical properties
Molecular weight1611.98
SolventDMSO
Spectral properties
Absorbance (nm)541
Correction factor (260 nm)0.634
Correction factor (280 nm)0.463
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)368
Emission (nm)560
Quantum yield
0.35
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 22, 2025