mFluor™ UV610 maleimide

mFluor™ dyes are engineered for advanced multicolor flow cytometry applications, offering significant Stokes Shifts and optimal excitation by standard flow cytometer laser lines, such as 350 nm, 405 nm, 488 nm, 532 nm, and 633 nm. The mFluor™ UV610 dye, which exhibits a bright red-orange fluorescence and moderate photostability, is ideally suited for excitation at both the 350 nm and 561 nm laser lines, with an emission maximum of ~609 nm. This maleimide derivative is commonly used for the conjugation of thiol groups on proteins, oligonucleotide thiophosphates, and low molecular weight ligands, producing conjugates that exhibit significantly brighter fluorescence and enhanced photostability compared to those formed with other spectrally similar fluorophores.

<b>Top)</b> The spectral pattern was obtained using a 4-laser spectral cytometer. Lasers at wavelengths of 355 nm, 405 nm, 488 nm, and 640 nm were spatially offset to generate four distinct emission profiles. The combination of these profiles resulted in the overall spectral signature. <b>Bottom)</b> Flow cytometry analysis was conducted on whole blood cells stained with the CD4-mFluor™ UV610 conjugate. The fluorescence signal was detected using an Aurora spectral flow cytometer, specifically in the UV10-A channel optimized for mFluor™ UV610.

Protocol

SDS

Catalog	Size	Price	Quantity
1610	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ UV610 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	76.197 µL	380.984 µL	761.969 µL	3.81 mL	7.62 mL
5 mM	15.239 µL	76.197 µL	152.394 µL	761.969 µL	1.524 mL
10 mM	7.62 µL	38.098 µL	76.197 µL	380.984 µL	761.969 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1312.39 g/mol	=	mL	x	mM	=	mmol

References

View all 2 references: Citation Explorer

Quantification of Low Copy Number Proteins in Single Cells.

Authors:

Shi, Meng and Geng, Xuhui and Wang, Chengye and Guan, Yafeng

Journal:

Analytical chemistry (2019): 11493-11496

Fluorochrome choices for multi-color flow cytometry.

Authors:

Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto

Journal:

Journal of immunological methods (2019): 112618

Physical properties

Molecular weight	1312.39
Solvent	DMSO

Spectral properties

Absorbance (nm)	589
Correction factor (260 nm)	0.949
Correction factor (280 nm)	0.904
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	369
Emission (nm)	609
Quantum yield	0.25

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on April 4, 2026