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mFluor™ Violet 450 acid
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Blue™ labeling dye. mFluor™ Violet 450 acid is stable, and it is a common precursor for making a variety of mFluor™ Violet 450 derivatives.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11405 mg
Price
 
Physical properties
Molecular weight542.51
SolventDMSO
Spectral properties
Absorbance (nm)406
Correction factor (260 nm)0.338
Correction factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)
35000
1
Excitation (nm)406
Emission (nm)445
Quantum yield
0.81
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 12, 2025