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mFluor™ Violet 450-PEG4-Biotin Conjugate

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Physical properties
Molecular weight943.04
SolventDMSO
Spectral properties
Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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mFluor™ UV 375 goat anti-mouse IgG (H+L)
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mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)
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mFluor™ Red 780 goat anti-rabbit IgG (H+L)
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Show More (148)

OverviewpdfSDSpdfProtocol


Molecular weight
943.04
Absorbance (nm)
406
Correction Factor (260 nm)
0.338
Correction Factor (280 nm)
0.078
Extinction coefficient (cm -1 M -1)
350001
Excitation (nm)
406
Emission (nm)
445
Quantum yield
0.811
mFluor™ Violet 450-PEG4-Biotin Conjugate contains mFluor™ Violet 450 fluorophore that is well excited by the common 405 nm violet laser. It demonstrates excellent avidin binding and strong blue fluorescence. Its avidin-binding properties is similar to the native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 450 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 450-PEG4-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM106.04 µL530.2 µL1.06 mL5.302 mL10.604 mL
5 mM21.208 µL106.04 µL212.08 µL1.06 mL2.121 mL
10 mM10.604 µL53.02 µL106.04 µL530.2 µL1.06 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811

Images


References


View all 9 references: Citation Explorer
Cancer-Targeted Chitosan-Biotin-Conjugated Mesoporous Silica Nanoparticles as Carriers of Zinc Complexes to Achieve Enhanced Chemotherapy In Vitro and In Vivo.
Authors: Kundu, Bidyut Kumar and Pragti, and Carlton Ranjith, Wilson Alphonse and Shankar, Uday and Kannan, Rajaretinam Rajesh and Mobin, Shaikh M and Bandyopadhyay, Anasuya and Mukhopadhyay, Suman
Journal: ACS applied bio materials (2022): 190-204
High-avidity binding drives nucleation of amyloidogenic transthyretin monomer.
Authors: Gao, Li and Xie, Xinfang and Liu, Pan and Jin, Jing
Journal: JCI insight (2022)
Biotin Alone or a Science-Driven Nutraceutical Multi-Targeted Approach?
Authors: Callender, Valerie D. and Belpulsi, Danamarie
Journal: Journal of drugs in dermatology : JDD (2019): 952-953
Benzothiazole analogs as potential anti-TB agents: computational input and molecular dynamics.
Authors: Venugopala, Katharigatta N and Khedr, Mohammed A and Pillay, Melendhran and Nayak, Susanta K and Chandrashekharappa, Sandeep and Aldhubiab, Bandar E and Harsha, Sree and Attimard, Mahesh and Odhav, Bharti
Journal: Journal of biomolecular structure & dynamics (2019): 1830-1842
Synthesis of 6-PEtN-α-D-GalpNAc-(1->6)-β-D-Galp-(1->4)-β-D-GlcpNAc-(1->3)-β-D-Galp-(1->4)-β-D-Glcp, a Haemophilus influenzae lipopolysacharide structure, and biotin and protein conjugates thereof.
Authors: Sundgren, Andreas and Lahmann, Martina and Oscarson, Stefan
Journal: Beilstein journal of organic chemistry (2010): 704-8
Vibrational spectra and assignments using ab initio and density functional theory analysis on the structure of biotin.
Authors: Emami, Mohammad and Teimouri, Abbas and Chermahini, Alireza Najafi
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2008): 1516-24
Synthesis of a C-terminally biotinylated macrocyclic peptide mimetic exhibiting high Grb2 SH2 domain-binding affinity.
Authors: Shi, Zhen-Dan and Liu, Hongpeng and Zhang, Manchao and Worthy, Karen M and Bindu, Lakshman and Yang, Dajun and Fisher, Robert J and Burke, Terrence R
Journal: Bioorganic & medicinal chemistry (2005): 4200-8
Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)).
Authors: Rzepecki, Piotr W and Prestwich, Glenn D
Journal: The Journal of organic chemistry (2002): 5454-60
Estradiol-mimetic probes. Preparation of 17 alpha-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates.
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4