mFluor™ Violet 450-PEG4-Biotin Conjugate contains mFluor™ Violet 450 fluorophore that is well excited by the common 405 nm violet laser. It demonstrates excellent avidin binding and strong blue fluorescence. Its avidin-binding properties is similar to the native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 450 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

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Catalog Number	3116
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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	943.04
Solvent	DMSO

Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Spectrum

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References

View all 9 references: Citation Explorer

High-avidity binding drives nucleation of amyloidogenic transthyretin monomer.
Authors: Gao, Li and Xie, Xinfang and Liu, Pan and Jin, Jing
Journal: JCI insight (2022)

Cancer-Targeted Chitosan-Biotin-Conjugated Mesoporous Silica Nanoparticles as Carriers of Zinc Complexes to Achieve Enhanced Chemotherapy In Vitro and In Vivo.
Authors: Kundu, Bidyut Kumar and Pragti, and Carlton Ranjith, Wilson Alphonse and Shankar, Uday and Kannan, Rajaretinam Rajesh and Mobin, Shaikh M and Bandyopadhyay, Anasuya and Mukhopadhyay, Suman
Journal: ACS applied bio materials (2022): 190-204

Benzothiazole analogs as potential anti-TB agents: computational input and molecular dynamics.
Authors: Venugopala, Katharigatta N and Khedr, Mohammed A and Pillay, Melendhran and Nayak, Susanta K and Chandrashekharappa, Sandeep and Aldhubiab, Bandar E and Harsha, Sree and Attimard, Mahesh and Odhav, Bharti
Journal: Journal of biomolecular structure & dynamics (2019): 1830-1842

Biotin Alone or a Science-Driven Nutraceutical Multi-Targeted Approach?
Authors: Callender, Valerie D. and Belpulsi, Danamarie
Journal: Journal of drugs in dermatology : JDD (2019): 952-953

Synthesis of 6-PEtN-α-D-GalpNAc-(1->6)-β-D-Galp-(1->4)-β-D-GlcpNAc-(1->3)-β-D-Galp-(1->4)-β-D-Glcp, a Haemophilus influenzae lipopolysacharide structure, and biotin and protein conjugates thereof.
Authors: Sundgren, Andreas and Lahmann, Martina and Oscarson, Stefan
Journal: Beilstein journal of organic chemistry (2010): 704-8

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