mFluor™ Violet 450-PEG4-Biotin Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 943.04 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 943.04 | Absorbance (nm) 406 | Correction Factor (260 nm) 0.338 | Correction Factor (280 nm) 0.078 | Extinction coefficient (cm -1 M -1) 350001 | Excitation (nm) 406 | Emission (nm) 445 | Quantum yield 0.811 |
mFluor™ Violet 450-PEG4-Biotin Conjugate contains mFluor™ Violet 450 fluorophore that is well excited by the common 405 nm violet laser. It demonstrates excellent avidin binding and strong blue fluorescence. Its avidin-binding properties is similar to the native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 450 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 450-PEG4-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 106.04 µL | 530.2 µL | 1.06 mL | 5.302 mL | 10.604 mL |
| 5 mM | 21.208 µL | 106.04 µL | 212.08 µL | 1.06 mL | 2.121 mL |
| 10 mM | 10.604 µL | 53.02 µL | 106.04 µL | 530.2 µL | 1.06 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Images
Figure 1. mFluor™ Violet 450-PEG4-Biotin Conjugate contains mFluor™ Violet 450 fluorophore that is well excited by the common 405 nm violet laser. It demonstrates excellent avidin binding and strong blue fluorescence.
References
View all 9 references: Citation Explorer
Cancer-Targeted Chitosan-Biotin-Conjugated Mesoporous Silica Nanoparticles as Carriers of Zinc Complexes to Achieve Enhanced Chemotherapy In Vitro and In Vivo.
Authors: Kundu, Bidyut Kumar and Pragti, and Carlton Ranjith, Wilson Alphonse and Shankar, Uday and Kannan, Rajaretinam Rajesh and Mobin, Shaikh M and Bandyopadhyay, Anasuya and Mukhopadhyay, Suman
Journal: ACS applied bio materials (2022): 190-204
Authors: Kundu, Bidyut Kumar and Pragti, and Carlton Ranjith, Wilson Alphonse and Shankar, Uday and Kannan, Rajaretinam Rajesh and Mobin, Shaikh M and Bandyopadhyay, Anasuya and Mukhopadhyay, Suman
Journal: ACS applied bio materials (2022): 190-204
High-avidity binding drives nucleation of amyloidogenic transthyretin monomer.
Authors: Gao, Li and Xie, Xinfang and Liu, Pan and Jin, Jing
Journal: JCI insight (2022)
Authors: Gao, Li and Xie, Xinfang and Liu, Pan and Jin, Jing
Journal: JCI insight (2022)
Biotin Alone or a Science-Driven Nutraceutical Multi-Targeted Approach?
Authors: Callender, Valerie D. and Belpulsi, Danamarie
Journal: Journal of drugs in dermatology : JDD (2019): 952-953
Authors: Callender, Valerie D. and Belpulsi, Danamarie
Journal: Journal of drugs in dermatology : JDD (2019): 952-953
Benzothiazole analogs as potential anti-TB agents: computational input and molecular dynamics.
Authors: Venugopala, Katharigatta N and Khedr, Mohammed A and Pillay, Melendhran and Nayak, Susanta K and Chandrashekharappa, Sandeep and Aldhubiab, Bandar E and Harsha, Sree and Attimard, Mahesh and Odhav, Bharti
Journal: Journal of biomolecular structure & dynamics (2019): 1830-1842
Authors: Venugopala, Katharigatta N and Khedr, Mohammed A and Pillay, Melendhran and Nayak, Susanta K and Chandrashekharappa, Sandeep and Aldhubiab, Bandar E and Harsha, Sree and Attimard, Mahesh and Odhav, Bharti
Journal: Journal of biomolecular structure & dynamics (2019): 1830-1842
Synthesis of 6-PEtN-α-D-GalpNAc-(1->6)-β-D-Galp-(1->4)-β-D-GlcpNAc-(1->3)-β-D-Galp-(1->4)-β-D-Glcp, a Haemophilus influenzae lipopolysacharide structure, and biotin and protein conjugates thereof.
Authors: Sundgren, Andreas and Lahmann, Martina and Oscarson, Stefan
Journal: Beilstein journal of organic chemistry (2010): 704-8
Authors: Sundgren, Andreas and Lahmann, Martina and Oscarson, Stefan
Journal: Beilstein journal of organic chemistry (2010): 704-8
Vibrational spectra and assignments using ab initio and density functional theory analysis on the structure of biotin.
Authors: Emami, Mohammad and Teimouri, Abbas and Chermahini, Alireza Najafi
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2008): 1516-24
Authors: Emami, Mohammad and Teimouri, Abbas and Chermahini, Alireza Najafi
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2008): 1516-24
Synthesis of a C-terminally biotinylated macrocyclic peptide mimetic exhibiting high Grb2 SH2 domain-binding affinity.
Authors: Shi, Zhen-Dan and Liu, Hongpeng and Zhang, Manchao and Worthy, Karen M and Bindu, Lakshman and Yang, Dajun and Fisher, Robert J and Burke, Terrence R
Journal: Bioorganic & medicinal chemistry (2005): 4200-8
Authors: Shi, Zhen-Dan and Liu, Hongpeng and Zhang, Manchao and Worthy, Karen M and Bindu, Lakshman and Yang, Dajun and Fisher, Robert J and Burke, Terrence R
Journal: Bioorganic & medicinal chemistry (2005): 4200-8
Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)).
Authors: Rzepecki, Piotr W and Prestwich, Glenn D
Journal: The Journal of organic chemistry (2002): 5454-60
Authors: Rzepecki, Piotr W and Prestwich, Glenn D
Journal: The Journal of organic chemistry (2002): 5454-60
Estradiol-mimetic probes. Preparation of 17 alpha-(6-aminohexynyl)estradiol biotin, fluorescein and acridinium conjugates.
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4
Authors: Adamczyk, M and Chen, Y Y and Moore, J A and Mattingly, P G
Journal: Bioorganic & medicinal chemistry letters (1998): 1281-4