Name: mFluor™ Violet 470 SE
Brand: AAT Bioquest
SKU: 1225
Price: 237 USD
Availability: InStock

mFluor™ Violet 470 SE

mFluor™ Violet 470 SE is a large Stokes shift violet-excitable dye that minimizes spectral spillover while providing bright blue emission, ideal for complex multicolor flow cytometry experimental designs.

Large Stokes Shift (405→470 nm): Minimizes spectral overlap for improved multicolor flow cytometry resolution
Violet Laser Optimization: Bright emission with 405 nm excitation for modern cytometer compatibility
Spectral Flow Cytometry: Unique emission profile enables additional color channels in complex panels
Pacific Orange Alternative: Similar spectral properties with enhanced photostability

Reaction scheme of Dye-NHS ester for chemical conjugation with primary amines.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1225	1 mg	Price

Overview

mFluor™ Violet 470 is a unique color for multicolor flow cytometry applications. Its SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 470 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. mFluor™ Violet 470 dyes have fluorescence excitation and emission maxima of ~405 nm and ~470 nm respectively. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 470 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	153.196 µL	765.978 µL	1.532 mL	7.66 mL	15.32 mL
5 mM	30.639 µL	153.196 µL	306.391 µL	1.532 mL	3.064 mL
10 mM	15.32 µL	76.598 µL	153.196 µL	765.978 µL	1.532 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	652.76 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.

Authors:

Sahi, Maryam and Andersson, Sarah and Mattson, Cecilia and Dale, Matilda and Kagiolglou, Sofia and Hofström, Camilla and Persson, Helena and Klingström, Jonas and Chiodi, Francesca and Fredolini, Claudia

Journal:

Journal of visualized experiments : JoVE (2024)

Development of a Duplex Serological Multiplex Assay for the Simultaneous Detection of Epstein-Barr Virus IgA and IgG Antibodies in Nasopharyngeal Carcinoma Patients.

Authors:

Schieber, Jennifer and Pring, Miranda and Ness, Andy and Liu, Zhiwei and Hsu, Wan-Lun and Brenner, Nicole and Butt, Julia and Waterboer, Tim and Simon, Julia

Journal:

Cancers (2023)

Infection with Influenzavirus A in a murine model induces epithelial bronchial lesions and distinct waves of innate immune-cell recruitment.

Authors:

Rivière, Frédéric and Burger, Julien and Lefèvre, François and Garnier, Annabelle and Vigne, Clarisse and Tournier, Jean-Nicolas and Billon-Denis, Emmanuelle

Journal:

Frontiers in immunology (2023): 1241323

Anti-PD-1 immune-related adverse events are associated with high therapeutic antibody fixation on T cells.

Authors:

Gazzano, Marianne and Parizot, Christophe and Psimaras, Dimitri and Vozy, Aurore and Baron, Marine and Abbar, Baptiste and Fallet, Vincent and Litvinova, Elena and Canellas, Anthony and Birzu, Cristina and Pourcher, Valérie and Touat, Mehdi and Weiss, Nicolas and Demeret, Sophie and Roos-Weil, Damien and Spano, Jean-Philippe and Lebbe, Celeste and Salem, Joe-Elie and Cadranel, Jacques and Hervier, Baptiste and Gorochov, Guy and Guihot, Amélie

Journal:

Frontiers in immunology (2022): 1082084

iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility.

Authors:

Jensen, Holly A and Kim, Jeong

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)

Physical properties

Molecular weight	652.76
Solvent	DMSO

Spectral properties

Excitation (nm)	432
Emission (nm)	479

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 15, 2026