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AAT Bioquest

mFluor™ Violet 490 SE

Product key features

  • Unique fluorescence spectra
  • Large Stokes Shift
  • Optimally excited by violet laser
  • Robust and convenient to use

Product description

mFluor™ Violet 490 is a unique color for multicolor flow cytometry applications. Its SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 490 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. mFluor™ Violet 490 dyes have fluorescence excitation close to the violet laser (at ~405 nm) with emission at ~490 nm respectively. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
mFluor™ Violet 430 SE411426700001-0.17-
mFluor™ Violet 450 SE4064453500010.8110.0780.338
mFluor™ Violet 480 SE40447540,0000.62510.65701.23
mFluor™ Violet 500 SE4105012500010.8110.3650.769
mFluor™ Violet 505 SE3935044000010.4510.4030.888
mFluor™ Violet 510 SE4125052500010.8610.3660.464
mFluor™ Violet 530 SE505525200001---
mFluor™ Violet 540 SE4025351800010.2110.5431.326
mFluor™ Violet 545 SE3935432000010.1510.4961.08
mFluor™ Violet 550 SE5275509000010.3110.3060.474
mFluor™ Violet 590 SE5645919000010.2210.3290.632
mFluor™ Violet 610 SE5966129000010.310.660.532
Show More (3)

References

View all 50 references: Citation Explorer
Highly Air-Stable N-Doped Two-Dimensional Violet Phosphorus with Atomically Flat Surfaces.
Authors: He, Qingyuan and Wang, Dan-Dong and Qiu, Haixin and Si, Nan and Yuan, Qinglin and Wang, Rui and Liu, Siyu and Wang, Yanming
Journal: ACS nano (2025): 427-438
Physical-Chemical Assessment of Azo Dye Basic Violet I (BVI) Discoloration Using the Corona Plasma in Batch and Flow Systems.
Authors: Gomez, Aaron and Rodríguez Albarrán, María José and Vergara Sanchez, Josefina and Torres, Cesar and Osorio, Daniel and Martínez, Horacio and Saldarriaga, Hugo and Reyes, Pedro Guillermo
Journal: ACS omega (2024): 8037-8047
A critical review of ultra-violet light emitting diodes as a one water disinfection technology.
Authors: Rauch, Kyle D and MacIsaac, Sean A and Reid, Bailey and Mullin, Toni J and Atkinson, Ariel J and Pimentel, Anthony L and Stoddart, Amina K and Linden, Karl G and Gagnon, Graham A
Journal: Water research X (2024): 100271
Modified cell trace violet proliferation assay preserves lymphocyte viability and allows spectral flow cytometry analysis.
Authors: Davis, Joanne E and Ludford-Menting, Mandy and Koldej, Rachel and Ritchie, David S
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2024): 394-403
A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay.
Authors: de la Fuente-Jiménez, Juan Luis and Rodríguez-Rivas, César Iván and Mitre-Aguilar, Irma Beatriz and Torres-Copado, Andrea and García-López, Eric Alejandro and Herrera-Celis, José and Arvizu-Espinosa, María Goretti and Garza-Navarro, Marco Antonio and Arriaga, Luis Gerardo and García, Janet Ledesma and García-Gutiérrez, Domingo Ixcóatl and Dehesa, Alejandro Zentella and Sharma, Ashutosh and Oza, Goldie
Journal: International journal of molecular sciences (2023)
Page updated on June 28, 2025

Ordering information

Price
Unit size
Catalog Number1226
Quantity
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Additional ordering information

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Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Physical properties

Molecular weight

672.71

Solvent

DMSO

Spectral properties

Excitation (nm)

385

Emission (nm)

488

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure