Name: mFluor™ Violet 490 SE
Brand: AAT Bioquest
SKU: 1226
Price: 218 USD
Availability: InStock

mFluor™ Violet 490 SE

mFluor™ Violet 490 SE is a unique violet-excited fluorophore with exceptionally large Stokes shift that enables multiplexing with other violet dyes while maintaining bright signal for flow cytometry applications.

Exceptional Stokes shift: ~100 nm separation between excitation and emission reduces self-quenching
Violet Laser Specific: Designed for 405 nm excitation with minimal cross-excitation from other lasers
Multiparameter Compatibility: Enables use with other violet dyes without spectral compensation issues
Enhanced Protein Labeling: Optimized NHS ester reactivity for antibodies over 10 kDa

Schematic illustrating the NHS ester–amine coupling between an NHS ester‐activated dye and an amine‐modified biomolecule. NHS esters react readily with lysine side‐chain amines or N‐terminal amines on proteins, peptides, and amine‐modified oligonucleotides under mild conditions (pH 7–9). The resulting amide bond is stable and physiologically compatible.

Catalog	Size	Price	Quantity
1226	1 mg	Price

Overview

mFluor™ Violet 490 is a unique color for multicolor flow cytometry applications. Its SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 490 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. mFluor™ Violet 490 dyes have fluorescence excitation close to the violet laser (at ~405 nm) with emission at ~490 nm respectively. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 490 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.412 µL	857.06 µL	1.714 mL	8.571 mL	17.141 mL
5 mM	34.282 µL	171.412 µL	342.824 µL	1.714 mL	3.428 mL
10 mM	17.141 µL	85.706 µL	171.412 µL	857.06 µL	1.714 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	583.39 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Highly Air-Stable N-Doped Two-Dimensional Violet Phosphorus with Atomically Flat Surfaces.

Authors:

He, Qingyuan and Wang, Dan-Dong and Qiu, Haixin and Si, Nan and Yuan, Qinglin and Wang, Rui and Liu, Siyu and Wang, Yanming

Journal:

ACS nano (2025): 427-438

Physical-Chemical Assessment of Azo Dye Basic Violet I (BVI) Discoloration Using the Corona Plasma in Batch and Flow Systems.

Authors:

Gomez, Aaron and Rodríguez Albarrán, María José and Vergara Sanchez, Josefina and Torres, Cesar and Osorio, Daniel and Martínez, Horacio and Saldarriaga, Hugo and Reyes, Pedro Guillermo

Journal:

ACS omega (2024): 8037-8047

A critical review of ultra-violet light emitting diodes as a one water disinfection technology.

Authors:

Rauch, Kyle D and MacIsaac, Sean A and Reid, Bailey and Mullin, Toni J and Atkinson, Ariel J and Pimentel, Anthony L and Stoddart, Amina K and Linden, Karl G and Gagnon, Graham A

Journal:

Water research X (2024): 100271

Modified cell trace violet proliferation assay preserves lymphocyte viability and allows spectral flow cytometry analysis.

Authors:

Davis, Joanne E and Ludford-Menting, Mandy and Koldej, Rachel and Ritchie, David S

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2024): 394-403

A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay.

Authors:

de la Fuente-Jiménez, Juan Luis and Rodríguez-Rivas, César Iván and Mitre-Aguilar, Irma Beatriz and Torres-Copado, Andrea and García-López, Eric Alejandro and Herrera-Celis, José and Arvizu-Espinosa, María Goretti and Garza-Navarro, Marco Antonio and Arriaga, Luis Gerardo and García, Janet Ledesma and García-Gutiérrez, Domingo Ixcóatl and Dehesa, Alejandro Zentella and Sharma, Ashutosh and Oza, Goldie

Journal:

International journal of molecular sciences (2023)

Physical properties

Molecular weight	583.39
Solvent	DMSO

Spectral properties

Excitation (nm)	385
Emission (nm)	488

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on April 15, 2026