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mFluor™ Violet 500 SE
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green™ (ThermoFisher) and BD Horizon™ V500 labeling dyes. mFluor™ Violet 500 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 500 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor™ Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green™ (ThermoFisher) and BD Horizon™ V500 with much stronger absorption, making the mFluor™ Violet 500 conjugates much more sensitive.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 500 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 500 specific V5-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 500 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 500 specific V5-A channel.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
11491 mg
Price
 
Physical properties
Molecular weight672.71
SolventDMSO
Spectral properties
Absorbance (nm)412
Correction factor (260 nm)0.769
Correction factor (280 nm)0.365
Extinction coefficient (cm -1 M -1)
25000
1
Excitation (nm)410
Emission (nm)501
Quantum yield
0.81
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
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Page updated on October 21, 2025