mFluor™ Violet 500 SE

mFluor™ Violet 500 SE is a violet-excitable, amine-reactive fluorescent dye for labeling antibodies and other proteins for multicolor flow cytometry applications.

Spectral Properties: Excitation at 410 nm and emission at 501 nm.
Reaction Chemistry: Succinimidyl ester reacts selectively with protein amino groups to form stable conjugates.
Fluorescence Brightness: Produces brighter conjugates than Pacific Green™ and BD Horizon™ V500 under comparable conditions.
Applications: Suitable for labeling monoclonal antibodies, polyclonal antibodies, and proteins larger than 10 kDa for violet-laser flow cytometry.

<strong>Top)</strong> Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature. <strong>Bottom)</strong> Flow cytometry analysis of whole blood cells stained with CD4-mFluor™ Violet 500 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 500 specific V5-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
1149	1 mg	Price

Overview

AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green™ (ThermoFisher) and BD Horizon™ V500 labeling dyes. mFluor™ Violet 500 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 500 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor™ Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green™ (ThermoFisher) and BD Horizon™ V500 with much stronger absorption, making the mFluor™ Violet 500 conjugates much more sensitive.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 500 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	148.652 µL	743.262 µL	1.487 mL	7.433 mL	14.865 mL
5 mM	29.73 µL	148.652 µL	297.305 µL	1.487 mL	2.973 mL
10 mM	14.865 µL	74.326 µL	148.652 µL	743.262 µL	1.487 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	672.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra and Martens, Erik and Liekens, S and ra and Noppen, Sam and Damme, Jo Van and others

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	672.71
Solvent	DMSO

Spectral properties

Absorbance (nm)	412
Correction factor (260 nm)	0.769
Correction factor (280 nm)	0.365
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	410
Emission (nm)	501
Quantum yield	0.81 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 12, 2026