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mFluor™ Violet 510 acid
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 510 dyes have fluorescence excitation and emission maxima of ~405 nm and ~510 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Orange™ labeling dye. mFluor™ Violet 510 acid is stable, and it is a common precursor for making a variety of mFluor™ Violet 510 derivatives.
<p>With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>
<p>With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11415 mg
Price
 
Physical properties
Molecular weight~500
SolventDMSO
Spectral properties
Absorbance (nm)410
Correction factor (260 nm)0.464
Correction factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)
25000
1
Excitation (nm)412
Emission (nm)505
Quantum yield
0.86
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 3, 2025