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mFluor™ Violet 540 SE
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser liness of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 540 dyes have fluorescence excitation and emission maxima of ~405 nm and ~540 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Orange™ labeling dye. mFluor™ Violet 540 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 540 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 540 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 540 specific V7-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 540 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 540 specific V7-A channel.
CatalogSize
Price
Quantity
11521 mg
Price
 
Physical properties

Molecular weight1535.88
SolventDMSO
Spectral properties

Absorbance (nm)401
Correction factor (260 nm)1.326
Correction factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)
18000
1
Excitation (nm)402
Emission (nm)535
Quantum yield
0.21
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 3, 2025