mFluor™ Violet 540-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluor™ Violet 540-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ Violet 540 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 405 nm excitation (e.g., the Violet Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

<p>Flow cytometry analysis of HL-60 cells stained with(Red)  or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin and then followed by mFluor™ Violet 540-streptavidin conjugate (Cat#16932). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the Pacific Orange channel.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16932	100 ug	Price

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Authors:

Largy E, Hamon F, Teulade-Fichou MP.

Journal:

Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots

Authors:

Mittal R, Bruchez MP.

Journal:

Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities

Authors:

Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.

Journal:

J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

Authors:

Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.

Journal:

BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Authors:

Sun M, Du L, Gao S, Bao Y, Wang S.

Journal:

Steroids (2010): 400

Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Absorbance (nm)	401
Correction factor (260 nm)	1.326
Correction factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000 ¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 4, 2025