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mFluor™ Violet 540 Styramide
The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor™ dyes, Styramide™ conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide™ radicals over tyramide ensures faster, more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor™ Violet 540 Styramide uses the bright green fluorescent dye mFluor™ Violet 540 (Ex/Em = 402/535 nm) to label targets in situ. mFluor™ Violet 540 is well-excited by the 405 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and red fluorescent probes or other spectrally compatible Styramide conjugates and PSA™ Imaging Kits.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Violet 540 styramide and detected with a Violet filter set.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Violet 540 styramide and detected with a Violet filter set.
CatalogSize
Price
Quantity
45077100 Slides
Price
 
Physical properties

SolventDMSO
Spectral properties

Absorbance (nm)401
Correction factor (260 nm)1.326
Correction factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)
18000
1
Excitation (nm)402
Emission (nm)535
Quantum yield
0.21
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings

Fluorescence microscope
Excitation402 nm
Emission535 nm
Recommended plateBlack wall/clear bottom
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Page updated on October 5, 2025