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mFluor™ Violet 540 Styramide
The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor™ dyes, Styramide™ conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide™ radicals over tyramide ensures faster, more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor™ Violet 540 Styramide uses the bright green fluorescent dye mFluor™ Violet 540 (Ex/Em = 402/535 nm) to label targets in situ. mFluor™ Violet 540 is well-excited by the 405 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and red fluorescent probes or other spectrally compatible Styramide conjugates and PSA™ Imaging Kits.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Violet 540 styramide and detected with a Violet filter set.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Violet 540 styramide and detected with a Violet filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
45077100 Slides
Price
 
Physical properties
SolventDMSO
Spectral properties
Absorbance (nm)401
Correction factor (260 nm)1.326
Correction factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)
18000
1
Excitation (nm)402
Emission (nm)535
Quantum yield
0.21
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
Excitation402 nm
Emission535 nm
Recommended plateBlack wall/clear bottom
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Page updated on October 12, 2025