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mFluor™ Violet 550 SE
mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet dyes are optimized to be excited with a violet laser at 405 nm. AAT Bioquest offers the largest collection of fluorescent dyes that are excited by violet laser. mFluor™ Violet 550 dyes have fluorescence excitation and emission maxima of ~405 nm and ~550 nm respectively. These spectral characteristics make them a unique color for flow cytometry application. mFluor™ Violet 550 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 550 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 550 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 550 specific V7-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 550 conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor&trade; Violet 550 specific V7-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11531 mg
Price
 
Physical properties
Molecular weight1125.2
SolventDMSO
Spectral properties
Absorbance (nm)527
Correction factor (260 nm)0.474
Correction factor (280 nm)0.306
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)420
Emission (nm)550
Quantum yield
0.31
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025