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mFluor™ Violet 550-streptavidin conjugate

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Fax1-800-609-2943
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Physical properties
Molecular weight~52 kDa
SolventWater
Spectral properties
Absorbance (nm)527
Correction Factor (260 nm)0.474
Correction Factor (280 nm)0.306
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)527
Emission (nm)550
Quantum yield0.311
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDS


Molecular weight
~52 kDa
Absorbance (nm)
527
Correction Factor (260 nm)
0.474
Correction Factor (280 nm)
0.306
Extinction coefficient (cm -1 M -1)
900001
Excitation (nm)
527
Emission (nm)
550
Quantum yield
0.311
mFluor™ Violet 550-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 550 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 550 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 550 exhibits a bright and photostable emission of green fluorescence at 550 nm. mFluor™ Violet 550 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)527
Correction Factor (260 nm)0.474
Correction Factor (280 nm)0.306
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)527
Emission (nm)550
Quantum yield0.311

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate4064453500010.8110.3380.078
mFluor™ Violet 510-streptavidin conjugate4125052500010.8610.4640.366
mFluor™ Violet 540-streptavidin conjugate4025351800010.2111.3260.543
mFluor™ Violet 545-streptavidin conjugate3935432000010.1511.080.496
mFluor™ Violet 590-streptavidin conjugate5645919000010.2210.6320.329
mFluor™ Violet 610-streptavidin conjugate5946129000010.310.5320.66