Name: mFluor™ Violet 590-streptavidin conjugate
Brand: AAT Bioquest
SKU: 47260
Price: 115 USD
Availability: InStock

mFluor™ Violet 590-streptavidin conjugate

mFluor™ Violet 590-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 590 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 590 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 590 exhibits a bright and photostable emission of orange fluorescence at 591 nm. mFluor™ Violet 590 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.

Protocol

SDS

Catalog	Size	Price	Quantity
47260	100 ug	Price

References

View all 13 references: Citation Explorer

[A method of marking neurons on brain slices using patch clamp technique].

Authors:

Gao, Jie and Huang, Yan Yun and Zhang, Yu Tong and DU, Xiang Xin and Zhang, Li Na and Hao, Na and Guo, Xia and Li, Jian Guo and Zhang, Yu

Journal:

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (2021): 445-448

Bone morphogenetic protein-4 inhibits adult neurogenesis and is regulated by fractone-associated heparan sulfates in the subventricular zone.

Authors:

Mercier, Frederic and Douet, Vanessa

Journal:

Journal of chemical neuroanatomy (2014): 54-61

Fractone-heparan sulfates mediate BMP-7 inhibition of cell proliferation in the adult subventricular zone.

Authors:

Douet, Vanessa and Arikawa-Hirasawa, Eri and Mercier, Frederic

Journal:

Neuroscience letters (2012): 120-5

[Rapid detection of SOX2 gene by primed in situ labeling].

Authors:

Luo, Xi and Ding, Xianping and Chen, Lin and Quan, Qiang

Journal:

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (2012): 289-92

Imaging of P-glycoprotein of H69/VP small-cell lung cancer lines by scanning near-field optical microscopy and confocal laser microspectrofluorometer.

Authors:

Qiao, Weihong and Shang, Guangyi and Lei, Franck H and Trussardi-Regnier, Aurélie and Angiboust, Jean-F and Millot, Jean-M and Manfait, Michel

Journal:

Ultramicroscopy (2005): 330-5

Physical properties

Molecular weight	~52 kDa
Solvent	Water

Spectral properties

Absorbance (nm)	563
Correction factor (260 nm)	0.632
Correction factor (280 nm)	0.329
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	423
Emission (nm)	591
Quantum yield	0.22 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Flow Cytometry Fluorochromes

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Page updated on October 30, 2025