mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 590 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 590 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 590 exhibits a bright and photostable emission of orange fluorescence at 591 nm. mFluor™ Violet 590 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 450-streptavidin conjugate | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
mFluor™ Violet 510-streptavidin conjugate | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
mFluor™ Violet 545-streptavidin conjugate | 393 | 543 | 200001 | 0.151 | 1.08 | 0.496 |
mFluor™ Violet 550-streptavidin conjugate | 527 | 550 | 900001 | 0.311 | 0.474 | 0.306 |
mFluor™ Violet 610-streptavidin conjugate | 594 | 612 | 900001 | 0.31 | 0.532 | 0.66 |
References
View all 13 references: Citation Explorer
[A method of marking neurons on brain slices using patch clamp technique].
Authors: Gao, Jie and Huang, Yan Yun and Zhang, Yu Tong and DU, Xiang Xin and Zhang, Li Na and Hao, Na and Guo, Xia and Li, Jian Guo and Zhang, Yu
Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (2021): 445-448
Authors: Gao, Jie and Huang, Yan Yun and Zhang, Yu Tong and DU, Xiang Xin and Zhang, Li Na and Hao, Na and Guo, Xia and Li, Jian Guo and Zhang, Yu
Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (2021): 445-448
Bone morphogenetic protein-4 inhibits adult neurogenesis and is regulated by fractone-associated heparan sulfates in the subventricular zone.
Authors: Mercier, Frederic and Douet, Vanessa
Journal: Journal of chemical neuroanatomy (2014): 54-61
Authors: Mercier, Frederic and Douet, Vanessa
Journal: Journal of chemical neuroanatomy (2014): 54-61
Fractone-heparan sulfates mediate BMP-7 inhibition of cell proliferation in the adult subventricular zone.
Authors: Douet, Vanessa and Arikawa-Hirasawa, Eri and Mercier, Frederic
Journal: Neuroscience letters (2012): 120-5
Authors: Douet, Vanessa and Arikawa-Hirasawa, Eri and Mercier, Frederic
Journal: Neuroscience letters (2012): 120-5
[Rapid detection of SOX2 gene by primed in situ labeling].
Authors: Luo, Xi and Ding, Xianping and Chen, Lin and Quan, Qiang
Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (2012): 289-92
Authors: Luo, Xi and Ding, Xianping and Chen, Lin and Quan, Qiang
Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (2012): 289-92
Imaging of P-glycoprotein of H69/VP small-cell lung cancer lines by scanning near-field optical microscopy and confocal laser microspectrofluorometer.
Authors: Qiao, Weihong and Shang, Guangyi and Lei, Franck H and Trussardi-Regnier, Aurélie and Angiboust, Jean-F and Millot, Jean-M and Manfait, Michel
Journal: Ultramicroscopy (2005): 330-5
Authors: Qiao, Weihong and Shang, Guangyi and Lei, Franck H and Trussardi-Regnier, Aurélie and Angiboust, Jean-F and Millot, Jean-M and Manfait, Michel
Journal: Ultramicroscopy (2005): 330-5
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