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mFluor™ Violet 590-streptavidin conjugate

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Physical properties
Molecular weight~52 kDa
Spectral properties
Absorbance (nm)563
Correction Factor (260 nm)0.632
Correction Factor (280 nm)0.329
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)564
Emission (nm)591
Quantum yield0.221
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
~52 kDa
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
mFluor™ Violet 590-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 590 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 590 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 590 exhibits a bright and photostable emission of orange fluorescence at 591 nm. mFluor™ Violet 590 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.


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Spectral properties

Absorbance (nm)563
Correction Factor (260 nm)0.632
Correction Factor (280 nm)0.329
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)564
Emission (nm)591
Quantum yield0.221

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate4064453500010.8110.3380.078
mFluor™ Violet 510-streptavidin conjugate4125052500010.8610.4640.366
mFluor™ Violet 540-streptavidin conjugate4025351800010.2111.3260.543
mFluor™ Violet 545-streptavidin conjugate3935432000010.1511.080.496
mFluor™ Violet 550-streptavidin conjugate5275509000010.3110.4740.306
mFluor™ Violet 610-streptavidin conjugate5946129000010.310.5320.66


View all 13 references: Citation Explorer
[A method of marking neurons on brain slices using patch clamp technique].
Authors: Gao, Jie and Huang, Yan Yun and Zhang, Yu Tong and DU, Xiang Xin and Zhang, Li Na and Hao, Na and Guo, Xia and Li, Jian Guo and Zhang, Yu
Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (2021): 445-448
Bone morphogenetic protein-4 inhibits adult neurogenesis and is regulated by fractone-associated heparan sulfates in the subventricular zone.
Authors: Mercier, Frederic and Douet, Vanessa
Journal: Journal of chemical neuroanatomy (2014): 54-61
Fractone-heparan sulfates mediate BMP-7 inhibition of cell proliferation in the adult subventricular zone.
Authors: Douet, Vanessa and Arikawa-Hirasawa, Eri and Mercier, Frederic
Journal: Neuroscience letters (2012): 120-5
[Rapid detection of SOX2 gene by primed in situ labeling].
Authors: Luo, Xi and Ding, Xianping and Chen, Lin and Quan, Qiang
Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (2012): 289-92
Imaging of P-glycoprotein of H69/VP small-cell lung cancer lines by scanning near-field optical microscopy and confocal laser microspectrofluorometer.
Authors: Qiao, Weihong and Shang, Guangyi and Lei, Franck H and Trussardi-Regnier, Aurélie and Angiboust, Jean-F and Millot, Jean-M and Manfait, Michel
Journal: Ultramicroscopy (2005): 330-5
cFos immunoreactivity is enhanced with biotin amplification.
Authors: Berghorn, K A and Bonnett, J H and Hoffman, G E
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1994): 1635-42
Preferential incorporation of 3'-azido-2',3'-dideoxythymidine into telomeric DNA and Z-DNA-containing regions of Chinese hamster ovary cells.
Authors: Olivero, O A and Poirier, M C
Journal: Molecular carcinogenesis (1993): 81-8
A simple method for immunofluorescent double staining with primary antisera from the same species.
Authors: Würden, S and Homberg, U
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1993): 627-30
The direct binding of an HIV fragment with granulocytes from healthy subjects and infected patients.
Authors: Gabrilovich, D I and Kozich, A T and Moshnikov, S A and Pokrovsky, V V
Journal: Scandinavian journal of immunology (1992): 369-72
Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins.
Authors: Bankston, P W and Porter, G A and Milici, A J and Palade, G E
Journal: European journal of cell biology (1991): 187-95