mFluor™ Violet 545-streptavidin conjugate
Ordering information
Price | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~52 kDa |
Solvent | Water |
Spectral properties
Absorbance (nm) | 398 |
Correction Factor (260 nm) | 1.08 |
Correction Factor (280 nm) | 0.496 |
Extinction coefficient (cm -1 M -1) | 200001 |
Excitation (nm) | 393 |
Emission (nm) | 543 |
Quantum yield | 0.151 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDS |
Molecular weight ~52 kDa | Absorbance (nm) 398 | Correction Factor (260 nm) 1.08 | Correction Factor (280 nm) 0.496 | Extinction coefficient (cm -1 M -1) 200001 | Excitation (nm) 393 | Emission (nm) 543 | Quantum yield 0.151 |
The mFluor™ Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor™ Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor™ Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 398 |
Correction Factor (260 nm) | 1.08 |
Correction Factor (280 nm) | 0.496 |
Extinction coefficient (cm -1 M -1) | 200001 |
Excitation (nm) | 393 |
Emission (nm) | 543 |
Quantum yield | 0.151 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 450-streptavidin conjugate | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
mFluor™ Violet 510-streptavidin conjugate | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
mFluor™ Violet 550-streptavidin conjugate | 527 | 550 | 900001 | 0.311 | 0.474 | 0.306 |
mFluor™ Violet 590-streptavidin conjugate | 564 | 591 | 900001 | 0.221 | 0.632 | 0.329 |
mFluor™ Violet 610-streptavidin conjugate | 594 | 612 | 900001 | 0.31 | 0.532 | 0.66 |
References
View all 22 references: Citation Explorer
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Journal: Frontiers in microbiology (2021): 741863
Authors: Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas
Journal: Frontiers in microbiology (2021): 741863
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Authors: Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi
Journal: International journal of nanomedicine (2015): 645-51
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Journal: Journal of cellular and molecular medicine (2011): 837-49
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Authors: Park, Jae-Chan and Kim, Jong-Man and Kwon, Oh-Joong and Lee, Kyoung-Ryul and Chai, Young-Guy and Oh, Heung-Bum
Journal: Journal of virological methods (2010): 269-75
Authors: Park, Jae-Chan and Kim, Jong-Man and Kwon, Oh-Joong and Lee, Kyoung-Ryul and Chai, Young-Guy and Oh, Heung-Bum
Journal: Journal of virological methods (2010): 269-75
In vivo detection of inducible nitric oxide synthase in rodent gliomas.
Authors: Towner, Rheal A and Smith, Nataliya and Doblas, Sabrina and Garteiser, Philippe and Watanabe, Yasuko and He, Ting and Saunders, Debra and Herlea, Oana and Silasi-Mansat, Robert and Lupu, Florea
Journal: Free radical biology & medicine (2010): 691-703
Authors: Towner, Rheal A and Smith, Nataliya and Doblas, Sabrina and Garteiser, Philippe and Watanabe, Yasuko and He, Ting and Saunders, Debra and Herlea, Oana and Silasi-Mansat, Robert and Lupu, Florea
Journal: Free radical biology & medicine (2010): 691-703
SNPs detection by a single-strand specific nuclease on a PNA zip-code microarray.
Authors: Mun, Hyo Young and Girigoswami, Agnishwar and Jung, Cheulhee and Cho, Dae-Yeon and Park, Hyun Gyu
Journal: Biosensors & bioelectronics (2009): 1706-11
Authors: Mun, Hyo Young and Girigoswami, Agnishwar and Jung, Cheulhee and Cho, Dae-Yeon and Park, Hyun Gyu
Journal: Biosensors & bioelectronics (2009): 1706-11
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Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2008): 515-20
Authors: Yu, Xiaobo and Zhao, Tianming and Sun, Zhidan and Yuan, Hongkun and He, Wei and Xu, Danke
Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2008): 515-20