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mFluor™ Violet 545-streptavidin conjugate

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Physical properties
Molecular weight~52 kDa
Spectral properties
Absorbance (nm)398
Correction Factor (260 nm)1.08
Correction Factor (280 nm)0.496
Extinction coefficient (cm -1 M -1)200001
Excitation (nm)393
Emission (nm)543
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
~52 kDa
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
The mFluor™ Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor™ Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor™ Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.


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Spectral properties

Absorbance (nm)398
Correction Factor (260 nm)1.08
Correction Factor (280 nm)0.496
Extinction coefficient (cm -1 M -1)200001
Excitation (nm)393
Emission (nm)543
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate4064453500010.8110.3380.078
mFluor™ Violet 510-streptavidin conjugate4125052500010.8610.4640.366
mFluor™ Violet 540-streptavidin conjugate4025351800010.2111.3260.543
mFluor™ Violet 550-streptavidin conjugate5275509000010.3110.4740.306
mFluor™ Violet 590-streptavidin conjugate5645919000010.2210.6320.329
mFluor™ Violet 610-streptavidin conjugate5946129000010.310.5320.66


View all 22 references: Citation Explorer
Low Concentrations of Chlorhexidine Inhibit the Formation and Structural Integrity of Enzyme-Treated Multispecies Oral Biofilms.
Authors: Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas
Journal: Frontiers in microbiology (2021): 741863
Protein immobilization onto electrochemically synthesized CoFe nanowires.
Authors: Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi
Journal: International journal of nanomedicine (2015): 645-51
In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.
Authors: Towner, Rheal A and Smith, Nataliya and Saunders, Debra and Henderson, Michael and Downum, Kristen and Lupu, Florea and Silasi-Mansat, Robert and Ramirez, Dario C and Gomez-Mejiba, Sandra E and Bonini, Marcelo G and Ehrenshaft, Marilyn and Mason, Ronald P
Journal: Diabetes (2012): 2405-13
Molecular MRI assessment of vascular endothelial growth factor receptor-2 in rat C6 gliomas.
Authors: He, Ting and Smith, Nataliya and Saunders, Debra and Doblas, Sabrina and Watanabe, Yasuko and Hoyle, Jessica and Silasi-Mansat, Robert and Lupu, Florea and Lerner, Megan and Brackett, Daniel J and Towner, Rheal A
Journal: Journal of cellular and molecular medicine (2011): 837-49
Sequence-dependent fluorescence of cyanine dyes on microarrays.
Authors: Agbavwe, Christy and Somoza, Mark M
Journal: PloS one (2011): e22177
Oligodendrocytes in mouse corpus callosum are coupled via gap junction channels formed by connexin47 and connexin32.
Authors: Maglione, Marta and Tress, Oliver and Haas, Brigitte and Karram, Khalad and Trotter, Jacqueline and Willecke, Klaus and Kettenmann, Helmut
Journal: Glia (2010): 1104-17
Development and clinical evaluation of a microarray for hepatitis C virus genotyping.
Authors: Park, Jae-Chan and Kim, Jong-Man and Kwon, Oh-Joong and Lee, Kyoung-Ryul and Chai, Young-Guy and Oh, Heung-Bum
Journal: Journal of virological methods (2010): 269-75
In vivo detection of inducible nitric oxide synthase in rodent gliomas.
Authors: Towner, Rheal A and Smith, Nataliya and Doblas, Sabrina and Garteiser, Philippe and Watanabe, Yasuko and He, Ting and Saunders, Debra and Herlea, Oana and Silasi-Mansat, Robert and Lupu, Florea
Journal: Free radical biology & medicine (2010): 691-703
SNPs detection by a single-strand specific nuclease on a PNA zip-code microarray.
Authors: Mun, Hyo Young and Girigoswami, Agnishwar and Jung, Cheulhee and Cho, Dae-Yeon and Park, Hyun Gyu
Journal: Biosensors & bioelectronics (2009): 1706-11
[A novel protein microarray detection technique based on biotin-avidin conjugation probe].
Authors: Yu, Xiaobo and Zhao, Tianming and Sun, Zhidan and Yuan, Hongkun and He, Wei and Xu, Danke
Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2008): 515-20