mFluor™ Violet 545-streptavidin conjugate

The mFluor™ Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor™ Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor™ Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
47250	100 ug	Price

References

View all 22 references: Citation Explorer

Low Concentrations of Chlorhexidine Inhibit the Formation and Structural Integrity of Enzyme-Treated Multispecies Oral Biofilms.

Authors:

Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas

Journal:

Frontiers in microbiology (2021): 741863

Protein immobilization onto electrochemically synthesized CoFe nanowires.

Authors:

Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi

Journal:

International journal of nanomedicine (2015): 645-51

In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.

Authors:

Towner, Rheal A and Smith, Nataliya and Saunders, Debra and Henderson, Michael and Downum, Kristen and Lupu, Florea and Silasi-Mansat, Robert and Ramirez, Dario C and Gomez-Mejiba, Sandra E and Bonini, Marcelo G and Ehrenshaft, Marilyn and Mason, Ronald P

Journal:

Diabetes (2012): 2405-13

Molecular MRI assessment of vascular endothelial growth factor receptor-2 in rat C6 gliomas.

Authors:

He, Ting and Smith, Nataliya and Saunders, Debra and Doblas, Sabrina and Watanabe, Yasuko and Hoyle, Jessica and Silasi-Mansat, Robert and Lupu, Florea and Lerner, Megan and Brackett, Daniel J and Towner, Rheal A

Journal:

Journal of cellular and molecular medicine (2011): 837-49

Sequence-dependent fluorescence of cyanine dyes on microarrays.

Authors:

Agbavwe, Christy and Somoza, Mark M

Journal:

PloS one (2011): e22177

Physical properties

Molecular weight	~52 kDa
Solvent	Water

Spectral properties

Absorbance (nm)	398
Correction factor (260 nm)	1.08
Correction factor (280 nm)	0.496
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	393
Emission (nm)	543
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025