mFluor™ Violet 510-streptavidin conjugate

Flow cytometry analysis of HL-60 cells stained with(Red)  or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin then followed by mFluor™ Violet 510-streptavidin conjugate (Cat#16931). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the AmCyan channel.

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Physical properties

Molecular weight	~52000
Solvent	Water

Spectral properties

Absorbance (nm)	410
Correction Factor (260 nm)	0.464
Correction Factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

~52000

Absorbance (nm)

410

Correction Factor (260 nm)

0.464

Correction Factor (280 nm)

0.366

Extinction coefficient (cm ^-1 M ^-1)

25000¹

Excitation (nm)

412

Emission (nm)

505

Quantum yield

0.86¹

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This mFluor™ Violet 510-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with mFluor™ Violet 510 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using a flow cytometer equiped with a 405 nm excitation (e.g., the Violet Laser). A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

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Spectral properties

Absorbance (nm)	410
Correction Factor (260 nm)	0.464
Correction Factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 450-streptavidin conjugate	406	445	35000¹	0.81¹	0.338	0.078
mFluor™ Violet 540-streptavidin conjugate	402	535	18000¹	0.21¹	1.326	0.543

Images

Figure 1. Flow cytometry analysis of HL-60 cells stained with(Red) or without (Green) 1ug/ml Anti-Human HLA-ABC-Biotin then followed by mFluor™ Violet 510-streptavidin conjugate (Cat#16931). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the AmCyan channel.

Citations

View all 1 citations: Citation Explorer

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer

A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)

Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362

Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400

Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385

Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517

Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576

Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103

Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73