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mFluor™ Violet 610 maleimide
mFluor™ dyes are engineered for advanced multicolor flow cytometry applications, offering significant Stokes Shifts and optimal excitation by standard flow cytometer laser lines, such as 350 nm, 405 nm, 488 nm, 532 nm, and 633 nm. The mFluor™ Violet 610 dye, which exhibits a bright red fluorescence and moderate photostability, is ideally suited for excitation at the 405 nm laser line, with an emission maximum of ~612 nm. This maleimide derivative is commonly used for the conjugation of thiol groups on proteins, oligonucleotide thiophosphates, and low molecular weight ligands, producing conjugates that exhibit significantly brighter fluorescence and enhanced photostability compared to those formed with other spectrally similar fluorophores.
<b>Top)</b> The spectral pattern was obtained using a 4-laser spectral cytometer. Lasers at wavelengths of 355 nm, 405 nm, 488 nm, and 640 nm were spatially offset to generate four distinct emission profiles. The combination of these profiles resulted in the overall spectral signature. <b>Bottom)</b> Flow cytometry analysis was conducted on whole blood cells stained with the CD4-mFluor™ Violet 610 conjugate. The fluorescence signal was detected using an Aurora spectral flow cytometer, specifically in the V10-A channel optimized for mFluor™ Violet 610.
<b>Top)</b> The spectral pattern was obtained using a 4-laser spectral cytometer. Lasers at wavelengths of 355 nm, 405 nm, 488 nm, and 640 nm were spatially offset to generate four distinct emission profiles. The combination of these profiles resulted in the overall spectral signature. <b>Bottom)</b> Flow cytometry analysis was conducted on whole blood cells stained with the CD4-mFluor™ Violet 610 conjugate. The fluorescence signal was detected using an Aurora spectral flow cytometer, specifically in the V10-A channel optimized for mFluor™ Violet 610.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16131 mg
Price
 
Physical properties
Molecular weight1116.13
SolventDMSO
Spectral properties
Absorbance (nm)594
Correction factor (260 nm)0.532
Correction factor (280 nm)0.66
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)421
Emission (nm)612
Quantum yield
0.3
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 21, 2025