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mFluor™ Violet 610 SE
Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry. Now with spectral flow cytometry analysis, researchers and scientists can investigate an increasing number of molecules of interest. However, the potential of spectral flow cytometry is severely limited by the availability of fluorescent labels and readouts. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications, in particular, for spectral fluorescence flow cytometry. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 350 nm, 405 nm, 488 nm and 633 nm). mFluor™ Violet 610 dyes are well excited with the Violet laser with a red emission ~610 nm. These spectral characteristics make them very unique labeling dyes for spectral fluorescence flow cytometric applications. mFluor™ Violet 610 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 610 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 610 conjugate. The fluorescence signal was monitored using a Cytek&reg; Aurora spectral flow cytometer in the mFluor&trade; Violet 610 specific V10-A channel.
<strong>Top)</strong>&nbsp;Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to generate four distinct emission profiles, then, when combined, yielded the overall spectral signature.&nbsp;<strong>Bottom)</strong>&nbsp;Flow cytometry analysis of whole blood cells stained with CD4-mFluor&trade; Violet 610 conjugate. The fluorescence signal was monitored using a Cytek&reg; Aurora spectral flow cytometer in the mFluor&trade; Violet 610 specific V10-A channel.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
11561 mg
Price
 
Physical properties
Molecular weight1293.46
SolventDMSO
Spectral properties
Absorbance (nm)594
Correction factor (260 nm)0.532
Correction factor (280 nm)0.66
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)421
Emission (nm)612
Quantum yield
0.3
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 14, 2025